Key features and details
- Rabbit polyclonal to IL-34
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IL-34 antibody
See all IL-34 primary antibodies
DescriptionRabbit polyclonal to IL-34
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Synthetic peptide corresponding to Human IL-34 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: Caco 2; PC3; HepG2
Previously labelled as Interleukin 34.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab75723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use at an assay dependent dilution. Detects a band of approximately 48 kDa (predicted molecular weight: 27 kDa).|
FunctionCytokine that promotes the differentiation and viability of monocytes and macrophages. Stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Ligand for colony-stimulating factor-1 receptor CSF1R.
Tissue specificityDetected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.
Sequence similaritiesBelongs to the IL-34 family.
- Information by UniProt
- C16orf77 antibody
- Chromosome 16 open reading frame 77 antibody
- FPT025 antibody
All lanes : Anti-IL-34 antibody (ab75723) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : PC3 (human prostate carcinoma cell line) Whole cell lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
The 48 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to IL-34.
ICC/IF image of ab75723 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75723, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab75723 has been referenced in 3 publications.
- Walker DG et al. Studies on Colony Stimulating Factor Receptor-1 and Ligands Colony Stimulating Factor-1 and Interleukin-34 in Alzheimer's Disease Brains and Human Microglia. Front Aging Neurosci 9:244 (2017). PubMed: 28848420
- Zwicker S et al. Interleukin 34: a new modulator of human and experimental inflammatory bowel disease. Clin Sci (Lond) 129:281-90 (2015). PubMed: 25896238
- Chemel M et al. Interleukin 34 expression is associated with synovitis severity in rheumatoid arthritis patients. Ann Rheum Dis : (2011). IHC ; Human . PubMed: 22039170