Product nameAnti-IL-34 antibody
See all IL-34 primary antibodies
DescriptionRabbit polyclonal to IL-34
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Synthetic peptide corresponding to Human IL-34 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: Caco 2; PC3; HepG2
Previously labelled as Interleukin 34.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab75723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use at an assay dependent dilution. Detects a band of approximately 48 kDa (predicted molecular weight: 27 kDa).|
FunctionCytokine that promotes the differentiation and viability of monocytes and macrophages. Stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Ligand for colony-stimulating factor-1 receptor CSF1R.
Tissue specificityDetected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.
Sequence similaritiesBelongs to the IL-34 family.
- Information by UniProt
- C16orf77 antibody
- Chromosome 16 open reading frame 77 antibody
- FPT025 antibody
All lanes : Anti-IL-34 antibody (ab75723) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : PC3 (human prostate carcinoma cell line) Whole cell lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
The 48 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to IL-34.
ICC/IF image of ab75723 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75723, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Walker DG et al. Studies on Colony Stimulating Factor Receptor-1 and Ligands Colony Stimulating Factor-1 and Interleukin-34 in Alzheimer's Disease Brains and Human Microglia. Front Aging Neurosci 9:244 (2017). Read more (PubMed: 28848420) »
- Zwicker S et al. Interleukin 34: a new modulator of human and experimental inflammatory bowel disease. Clin Sci (Lond) 129:281-90 (2015). Read more (PubMed: 25896238) »