Anti-IL-36R antibody (ab180894)
Key features and details
- Rabbit polyclonal to IL-36R
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-36R antibody
See all IL-36R primary antibodies -
Description
Rabbit polyclonal to IL-36R -
Host species
Rabbit -
Specificity
ab180894 will detect all three isoforms of IL1RL2. ab180894 is predicted to not cross-react with IL1 Receptor I or ST2. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IL-36R (internal sequence). (16 amino acid peptide; NP_003845).
Database link: Q9HB29 -
Positive control
- Human small intestine lysate and tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 65 kDa).
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 65 kDa). |
Target
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Function
Receptor for interleukin 1 family member 9 (IL1F9). Binding to the agonist leads to the activation of NF-kappa-B. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8808 Human
- Omim: 604512 Human
- SwissProt: Q9HB29 Human
- Unigene: 659863 Human
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Alternative names
- IL 1 receptor related protein 2 antibody
- IL 1Rrp2 antibody
- IL 36R antibody
see all
Images
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Lane 1 : Anti-IL-36R antibody (ab180894) at 1 µg/ml
Lane 2 : Anti-IL-36R antibody (ab180894) at 2 µg/ml
All lanes : Human small intestine lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 65 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling IL1RL2 with ab180894 at 5 μg/ml.
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Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling IL1RL2 with ab180894 at 20 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab180894 has been referenced in 4 publications.
- Dai C et al. Role of IL-36?/IL-36R Signaling in Corneal Innate Defense Against Candida albicans Keratitis. Invest Ophthalmol Vis Sci 62:10 (2021). PubMed: 33970198
- Murrieta-Coxca JM et al. Role of IL-36 Cytokines in the Regulation of Angiogenesis Potential of Trophoblast Cells. Int J Mol Sci 22:N/A (2020). PubMed: 33396613
- Li Q et al. Spinal IL-36γ/IL-36R participates in the maintenance of chronic inflammatory pain through astroglial JNK pathway. Glia 67:438-451 (2019). PubMed: 30578562
- Russell SE et al. IL-36a expression is elevated in ulcerative colitis and promotes colonic inflammation. Mucosal Immunol 9:1193-204 (2016). PubMed: 26813344