Product nameAnti-IL-3RA / CD123 antibody [6H6]
See all IL-3RA / CD123 primary antibodies
DescriptionMouse monoclonal [6H6] to IL-3RA / CD123
Tested applicationsSuitable for: ICC/IF, IP, Flow Cyt, WB, IHC-Frmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to IL-3RA/ CD123.
Previously labelled as IL3RA.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2
Concentration information loading...
PurityProtein G purified
Light chain typekappa
- Anti-IL-3RA / CD123 antibody [6H6], prediluted (PerCP/Cy5.5®) (ab155368)
- Anti-IL-3RA / CD123 antibody [6H6] (PerCP/Cy5.5®) (ab157332)
- Anti-IL-3RA / CD123 antibody [6H6] (Biotin) (ab21560)
- Anti-IL-3RA / CD123 antibody [6H6] (PE/Cy5®) (ab232697)
- Anti-IL-3RA / CD123 antibody [6H6] (PE/Cy7 ®) (ab233585)
- Anti-IL-3RA / CD123 antibody [6H6] (Allophycocyanin) (ab239280)
- Anti-IL-3RA / CD123 antibody [6H6] (Phycoerythrin) (ab93523)
- Anti-IL-3RA / CD123 antibody [6H6] (Allophycocyanin) (ab95781)
- Anti-IL-3RA / CD123 antibody [6H6] (FITC) (ab95782)
Our Abpromise guarantee covers the use of ab21562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
100 µl volume or 100 µl of whole blood.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use at an assay dependent concentration. Detects a band of approximately 70 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionThis is a receptor for interleukin-3.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 5 subfamily.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
- Information by UniProt
- CD123 antibody
- CD123 antigen antibody
- hIL 3Ra antibody
Human peripheral blood lymphocytes stained with ab21562 (y-axis) and CD45RO PE/Cy5® (x-axis)
Immunofluorescence analysis of Human peripheral blood mononuclear cells, staining IL3RA / CD123 with ab21562.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA in PBS) for 24 hours at 4°C. An AlexaFluor®488-conjugated goat anti-mouse polyclonal IgG (1/250) was used as the secondary antibody.
Anti-IL-3RA / CD123 antibody [6H6] (ab21562) at 1/1000 dilution + Mouse spleen whole cell extract at 100 µg with Milk, 30 minutes at 25°C at 5 %
Goat anti-mouse IgG (H+L), HRP conjugate at 1/4000 dilution
Developed using the ECL technique.
Exposure time: 15 minutes