Key features and details
- Rabbit monoclonal [EP1037Y] to IL-3RB
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IL-3RB antibody [EP1037Y]
See all IL-3RB primary antibodies
DescriptionRabbit monoclonal [EP1037Y] to IL-3RB
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Human
Synthetic peptide within Human IL-3RB (C terminal). The exact sequence is proprietary.
- TF1 cell lysate; Human lymphoma tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Purification notesSupplied as cell supernatant in storage buffer.
Our Abpromise guarantee covers the use of ab52609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Predicted molecular weight: 97 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionHigh affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 2 fibronectin type-III domains.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
- Information by UniProt
- Beta-C antibody
- CD131 antibody
- CDw131 antibody
Anti-IL-3RB antibody [EP1037Y] (ab52609) at 1/5000 dilution + TF1 cell lysate at 10 µg
HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 97 kDa
The band at 130 kDa is the possible glycosylated form of IL3RB.
Immunohistochemical staining of paraffin-embedded human lymphoma using at a dilution of 1/100-1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab52609 has been referenced in 2 publications.
- Medana IM et al. Erythropoietin and its receptors in the brainstem of adults with fatal falciparum malaria. Malar J 8:261 (2009). IHC-P ; Human . PubMed: 19930602
- Schroeder JT et al. Human basophils secrete IL-3: evidence of autocrine priming for phenotypic and functional responses in allergic disease. J Immunol 182:2432-8 (2009). PubMed: 19201898