Key features and details
- Rabbit polyclonal to IL-3RB (phospho Y593)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IL-3RB (phospho Y593) antibody
See all IL-3RB primary antibodies
DescriptionRabbit polyclonal to IL-3RB (phospho Y593)
Specificityab53695 detects endogenous levels of IL3RB only when phosphorylated at tyrosine 593.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human IL-3RB.
- Human brain tissue and RAW264.7 cell extracts treated with G-CSF (25ng/ml, 15mins).
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab53695 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab53695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Predicted molecular weight: 97 kDa.|
FunctionHigh affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 2 fibronectin type-III domains.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
- Information by UniProt
- Beta-C antibody
- CD131 antibody
- CDw131 antibody
ab53695 at 1/50 dilution staining IL3RB in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
All lanes : Anti-IL-3RB (phospho Y593) antibody (ab53695)
Lane 1 : HepG2 cells treated with EGF (200ng/ml, 15 minutes)
Lane 2 : HepG2 cells treated with EGF (200ng/ml, 15 minutes) with blocking phosphopeptide
Predicted band size: 97 kDa
ab53695 has not yet been referenced specifically in any publications.