Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab9622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 15 kDa. To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Detects a band of approximately 15 kDa.
ELISA Use at an assay dependent concentration. To detect hIL-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (1.50 ng/ml), a concentration of 0.03 - 0.05 µg/ml of this antibody is required.
Sandwich ELISA Use at an assay dependent concentration. Can be used as Detection antibody with recommended pair.

Target

  • Function
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
  • Involvement in disease
    Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similarities
    Belongs to the IL-4/IL-13 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell growth factor 1 antibody
    • B cell IgG differentiation factor antibody
    • B Cell Stimulatory Factor 1 antibody
    • B-cell stimulatory factor 1 antibody
    • BCGF 1 antibody
    • BCGF1 antibody
    • Binetrakin antibody
    • BSF-1 antibody
    • BSF1 antibody
    • IGG1 induction factor antibody
    • IL 4 antibody
    • IL-4 antibody
    • IL4 antibody
    • IL4_HUMAN antibody
    • Il4e12 antibody
    • Interleukin 4 antibody
    • Interleukin 4 variant 2 antibody
    • Interleukin 4, isoform 1 antibody
    • Interleukin-4 antibody
    • Lymphocyte stimulatory factor 1 antibody
    • MGC79402 antibody
    • Pitrakinra antibody
    see all

Images

References

This product has been referenced in:
  • Kaewkangsadan V  et al. Tumour-draining axillary lymph nodes in patients with large and locally advanced breast cancers undergoing neoadjuvant chemotherapy (NAC): the crucial contribution of immune cells (effector, regulatory) and cytokines (Th1, Th2) to immune-mediated tumour cell death induced by NAC. BMC Cancer 18:123 (2018). IHC-P ; Human . Read more (PubMed: 29390966) »
  • Kasashima S  et al. Inflammatory features, including symptoms, increased serum interleukin-6, and C-reactive protein, in IgG4-related vascular diseases. Heart Vessels N/A:N/A (2018). Read more (PubMed: 29931542) »
See all 17 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

A good alternative to tonsilis spleen and maybe also tissue from the digestive tract, for instance colon or small intestine.

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Tumor)
Specification
Tumor
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No

Abcam user community

Verified customer

Submitted Jan 16 2012

Question
Answer

DISCOUNT CODE:*** Expiration date: May 13, 2012 I am very pleased to hear you would like to test ab9622 in mouse. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for ab9622 in mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Normal human spleen and lymph node should be a good positive controls. The antigen retrieval is mentioned under the IHC-P image on the datasheet- sodium citrate buffer (pH 6.0)- and other main specifications of the protocol used.

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