Overview

  • Product name

  • Description

    Rabbit polyclonal to IL-4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Highly pure (>98%) recombinant rIL-4 (rat Interleukin-4)

  • Positive control

    • Recombinant rat IL4 protein (ab9812) can be used as a positive control in WB.

Properties

  • Form

    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    unknown
  • Light chain type

    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 22315633
ELISA Use a concentration of 0.5 µg/ml.
WB Use a concentration of 0.1 - 0.2 µg/ml.

Target

  • Function

    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
  • Involvement in disease

    Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similarities

    Belongs to the IL-4/IL-13 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • B cell growth factor 1 antibody
    • B cell IgG differentiation factor antibody
    • B Cell Stimulatory Factor 1 antibody
    • B-cell stimulatory factor 1 antibody
    • BCGF 1 antibody
    • BCGF1 antibody
    • Binetrakin antibody
    • BSF-1 antibody
    • BSF1 antibody
    • IGG1 induction factor antibody
    • IL 4 antibody
    • IL-4 antibody
    • IL4 antibody
    • IL4_HUMAN antibody
    • Il4e12 antibody
    • Interleukin 4 antibody
    • Interleukin 4 variant 2 antibody
    • Interleukin 4, isoform 1 antibody
    • Interleukin-4 antibody
    • Lymphocyte stimulatory factor 1 antibody
    • MGC79402 antibody
    • Pitrakinra antibody
    see all

Images

  • Immunohistochemical analysis of rat endometrial tissue, staining IL4 with ab9811.

    Antigen retrieval was by heat mediation in a citrate buffer (pH 6) followed by blocking with 1% BSA for 30 minutes. Sections were incubated with primary antibody (1/50) overnight at 4°C. An HRP-goat anti-rabbit IgG was used as the secondary antibody (1/200). Staining was detected using DAB.

References

This product has been referenced in:

  • Izumi S  et al. A histopathologic study of the controlling role of T cells on experimental periodontitis in rats. J Dent Sci 13:87-96 (2018). Read more (PubMed: 30895102) »
  • Wu J  et al. Roles of programmed death protein 1/programmed death-ligand 1 in secondary brain injury after intracerebral hemorrhage in rats: selective modulation of microglia polarization to anti-inflammatory phenotype. J Neuroinflammation 14:36 (2017). WB ; Rat . Read more (PubMed: 28196545) »
See all 7 Publications for this product

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