Key features and details
- Rabbit polyclonal to IL-4 (Biotin)
- Suitable for: Sandwich ELISA, WB
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-IL-4 antibody (Biotin)
See all IL-4 primary antibodies
DescriptionRabbit polyclonal to IL-4 (Biotin)
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Mouse
Recombinant full length protein corresponding to Mouse IL-4.
FormLyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
Purification notesab84269 is affinity purified and sterile filtered.
Our Abpromise guarantee covers the use of ab84269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 mg/ml.
Allows the detection of at least 0.2 - 0.4 ng/well of recombinant mouse IL4.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 16 kDa.
The detection limit for recombinant mouse IL4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionParticipates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
Involvement in diseaseGenetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Sequence similaritiesBelongs to the IL-4/IL-13 family.
- Information by UniProt
- B cell growth factor 1 antibody
- B cell IgG differentiation factor antibody
- B Cell Stimulatory Factor 1 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab84269 has not yet been referenced specifically in any publications.