Overview

  • Product name
    Anti-IL-4 antibody [BVD4-1D11]
    See all IL-4 primary antibodies
  • Description
    Rat monoclonal [BVD4-1D11] to IL-4
  • Host species
    Rat
  • Specificity
    Recognises natural and recombinant mouse IL4. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Mouse IL-4.

  • General notes
    Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.
ELISA Use a concentration of 2 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 16.8 kDa.

Target

  • Function
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
  • Involvement in disease
    Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similarities
    Belongs to the IL-4/IL-13 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell growth factor 1 antibody
    • B cell IgG differentiation factor antibody
    • B Cell Stimulatory Factor 1 antibody
    • B-cell stimulatory factor 1 antibody
    • BCGF 1 antibody
    • BCGF1 antibody
    • Binetrakin antibody
    • BSF-1 antibody
    • BSF1 antibody
    • IGG1 induction factor antibody
    • IL 4 antibody
    • IL-4 antibody
    • IL4 antibody
    • IL4_HUMAN antibody
    • Il4e12 antibody
    • Interleukin 4 antibody
    • Interleukin 4 variant 2 antibody
    • Interleukin 4, isoform 1 antibody
    • Interleukin-4 antibody
    • Lymphocyte stimulatory factor 1 antibody
    • MGC79402 antibody
    • Pitrakinra antibody
    see all

Images

  • Anti-IL-4 antibody [BVD4-1D11] (ab11524) + mouse IL-4 recombinant protein

    Predicted band size: 16.8 kDa

References

This product has been referenced in:
  • García Nores GD  et al. CD4+ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema. Nat Commun 9:1970 (2018). Read more (PubMed: 29773802) »
  • Wu S  et al. Anti-asthmatic effect of pitavastatin through aerosol inhalation is associated with CD4+ CD25+ Foxp3+ T cells in an asthma mouse model. Sci Rep 7:6084 (2017). Read more (PubMed: 28729731) »
See all 9 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

Thank you for contacting us.

In this particular case, since you would be using the product in an application and on a species which we guarantee I would not be able to provide a testing discount.

This product is covered by our Abpromise. This guarantees that the ab11524 will work in the WB on mouse, or we will offer a replacement, credit, or refund within 6 months of purchase.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Answer

Thank you for your new contact. I am sorry for my delayed response but was out of office yesterday.

The immunogen was a recombinant full length mouse protein. As far as we are aware, the epitope has not been mapped yet so we do not have this information.

If you need any further assistance in the future, please do not hesitate to contact me.

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Question

I am sending to you in attachment the word document with answers.
Best regards,
1) Abcam product code ab
Ab11524
2) Abcam order reference number or product batch number

3) Description of the problem
Even with a high concentration (1/40), I can’t detect the protein, Ihave also a high background level and some unspecific interaction with the tissue
4) Sample preparation:
Species mouse
Type of sample: Fresh frozen sections,

5) Fixation step
Yes
If yes: Fixative agent and concentration : Acetone
Fixation time: 10 minutes
Fixation temperature: Cool 4°C

6) Antigen retrieval method

7) Permeabilization method:
Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers? YES and NO, I tried the both
Permeabilizing agent and concentration: Triton 0,1%


8) Blocking agent (eg BSA, serum…):
Concentration BSA 1%, GOAT NORMAL SERUM 5%
Blocking time 1H
Blocking temperature RT
Remark: Blocking and Permeabilisation are done in the same time and they are maintained even during the primary and secondary antibody incubation

9) Endogenous peroxidases blocked? no
Endogenous biotins blocked? no

10) Primary antibody (If more than one was used, describe in “additional notes”) :
Concentration or dilution 1/40
Diluent buffer PBS
Incubation time O/N 4°C

11) Secondary antibody:
Species: Goat
Reacts against: Rat
Concentration or dilution 1/400
Diluent buffer PBS
Incubation time 1H RT
Fluorochrome or enzyme conjugate Alexa Fluor 488

12) Washing after primary and secondary antibodies:
Buffer PBS
Number of washes 3 times

13) Detection method IF

14) How many times have you run this staining? 3 times
Do you obtain the same results every time? No
What steps have you altered to try and optimize the use of this antibody? Antibody concentration (1/100 à 1/40), Permeabilisation or not with Triton,

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Answer

Thank you for your enquiry regarding ab11524 and for taking the time to provide some useful details of the experiments.

I have read through the protocol and would make the following suggestions:

1) Try to block the non-specific bindings with 5% BSA (or 10% serum) in TBS-T and use it for diluting the primary and the secondary antibodies as well.

2) It may be worth diluting the primary antibody 1/200 and applying different fixative (methanol, ethanol) as possible. Normally fixation with acetone (ice-cold) would be no longer than 5 mins.

If you need any further assistance in the future, please do not hesitate to contact me.

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Question
Answer

Thank you very much for sending over the technical details.

Would it be possible to send me an image as well so that I can take a look at the signal?

I look forward to hearing from you soon.

Read More

Answer

Thank you for your enquiry regarding ab11524.

As the datasheet indicates the immunogne was a recombinant full length protein (Mouse). This antibody has been tested and characterized in different application such as Flow Cyt, IHC-Fr, ELISA, WB.

If you are happy to share the experimental details with me, I could take a look at the Protocol. Feel free to complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Answer

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Answer

Thank you for contacting us. We have about 30 products related to IL4 proteins. Could you please specify for which product you would be interested in getting more information. Please click on the following link to explore all anti IL4 antibodies; https://www.abcam.com/index.html?pageconfig=searchresults I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (atherosclerotic and aneurysmal lesions)
Specification
atherosclerotic and aneurysmal lesions
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Galina Sukhova

Verified customer

Submitted Mar 15 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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