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  1. Link

    il-4-antibody-epr1118y-ab62351.pdf

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Immunology Innate Immunity Cytokines Interleukins
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RecombinantRabMAb

Recombinant Anti-IL-4 antibody [EPR1118Y] (ab62351)

  • Datasheet
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Flow Cytometry (Intracellular) - Anti-IL-4 antibody [EPR1118Y] (ab62351)
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-4 antibody [EPR1118Y] (ab62351)
  • Western blot - Anti-IL-4 antibody [EPR1118Y] (ab62351)
  • Anti-IL-4 antibody [EPR1118Y] (ab62351)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1118Y] to IL-4
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

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Overview

  • Product name

    Anti-IL-4 antibody [EPR1118Y]
    See all IL-4 primary antibodies
  • Description

    Rabbit monoclonal [EPR1118Y] to IL-4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IL-4 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human tonsil lysate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1118Y
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-IL-4 antibody [EPR1118Y] (ab211374)
    • Anti-IL-4 antibody [EPR1118Y] - BSA and Azide free (ab246802)
    • Anti-IL-4 antibody [EPR1118Y] - Low endotoxin, Azide free (ab246803)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant mouse IL-4 protein (Active) (ab9729)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab62351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
1/1000 - 1/2000. Predicted molecular weight: 17 kDa.
ICC/IF
1/500.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
1/1000 - 1/2000. Predicted molecular weight: 17 kDa.
ICC/IF
1/500.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
  • Involvement in disease

    Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similarities

    Belongs to the IL-4/IL-13 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P05112 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3565 Human
    • Omim: 147780 Human
    • SwissProt: P05112 Human
    • Unigene: 73917 Human
    • Alternative names

      • B cell growth factor 1 antibody
      • B cell IgG differentiation factor antibody
      • B Cell Stimulatory Factor 1 antibody
      • B-cell stimulatory factor 1 antibody
      • BCGF 1 antibody
      • BCGF1 antibody
      • Binetrakin antibody
      • BSF-1 antibody
      • BSF1 antibody
      • IGG1 induction factor antibody
      • IL 4 antibody
      • IL-4 antibody
      • IL4 antibody
      • IL4_HUMAN antibody
      • Il4e12 antibody
      • Interleukin 4 antibody
      • Interleukin 4 variant 2 antibody
      • Interleukin 4, isoform 1 antibody
      • Interleukin-4 antibody
      • Lymphocyte stimulatory factor 1 antibody
      • MGC79402 antibody
      • Pitrakinra antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Anti-IL-4 antibody [EPR1118Y] (ab62351)
      Flow Cytometry (Intracellular) - Anti-IL-4 antibody [EPR1118Y] (ab62351)

      Intracellular Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling IL4 with unpurified ab62351 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-IL-4 antibody [EPR1118Y] (ab62351)
      Immunocytochemistry/ Immunofluorescence - Anti-IL-4 antibody [EPR1118Y] (ab62351)

      Immunocytochemistry/Immunofluorescence analysis of RAMOS (human Burkitt's lymphoma) labelling IL4 with purified ab62351 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Control: PBS only

    • Western blot - Anti-IL-4 antibody [EPR1118Y] (ab62351)
      Western blot - Anti-IL-4 antibody [EPR1118Y] (ab62351)
      Anti-IL-4 antibody [EPR1118Y] (ab62351) at 1/2000 dilution + Human tonsil lysate at 10 µg

      Secondary
      goat anti rabbit HRP at 1/2000 dilution

      Predicted band size: 17 kDa
      Observed band size: 14 kDa why is the actual band size different from the predicted?

    • Anti-IL-4 antibody [EPR1118Y] (ab62351)
      Anti-IL-4 antibody [EPR1118Y] (ab62351)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (7)

    Publishing research using ab62351? Please let us know so that we can cite the reference in this datasheet.

    ab62351 has been referenced in 7 publications.

    • Becares N  et al. Immune Regulatory Mediators in Plasma from Patients With Acute Decompensation Are Associated With 3-Month Mortality. Clin Gastroenterol Hepatol 18:1207-1215.e6 (2020). PubMed: 31446184
    • Granier C  et al. Formation of somatosensory detour circuits mediates functional recovery following dorsal column injury. Sci Rep 10:10953 (2020). PubMed: 32616790
    • Tao S  et al. Linc00514 promotes breast cancer metastasis and M2 polarization of tumor-associated macrophages via Jagged1-mediated notch signaling pathway. J Exp Clin Cancer Res 39:191 (2020). PubMed: 32943090
    • Deng S  et al. Relationship Between Pregnancy and Acute Disseminated Encephalomyelitis: A Single-Case Study. Front Immunol 11:609476 (2020). PubMed: 33597947
    • Ganieva U  et al. Involvement of Transcription Factor 21 in the Pathogenesis of Fibrosis in Endometriosis. Am J Pathol 190:145-157 (2020). PubMed: 31610174
    • Qu S  et al. MicroRNA-194 reduces inflammatory response and human dermal microvascular endothelial cells permeability through suppression of TGF-ß/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria. J Cell Biochem 121:111-124 (2020). PubMed: 31190349
    • Xie N  et al. miR-320a upregulation contributes to the development of preeclampsia by inhibiting the growth and invasion of trophoblast cells by targeting interleukin 4. Mol Med Rep 20:3256-3264 (2019). PubMed: 31432141

    Customer reviews and Q&As

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