Recombinant Anti-IL-4 antibody [EPR1118Y] (ab62351)
![Flow Cytometry - Anti-IL-4 antibody [EPR1118Y] (ab62351)](https://www.abcam.com/ps/products/62/ab62351/Images/ab62351-275012-anti-il-4-antibody-epr1118y-flow-cytometry.jpg "Flow Cytometry - Anti-IL-4 antibody [EPR1118Y] (ab62351)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1118Y] to IL-4
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-IL-4 antibody [EPR1118Y]
See all IL-4 primary antibodies -
Description
Rabbit monoclonal [EPR1118Y] to IL-4 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human IL-4 aa 50-150. The exact sequence is proprietary.
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Positive control
- Human tonsil lysate
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1118Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
|
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| WB |
1/1000 - 1/2000. Predicted molecular weight: 17 kDa.
|
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| ICC/IF |
1/500.
|
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/2000. Predicted molecular weight: 17 kDa. |
|
ICC/IF
1/500. |
Target
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Function
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. -
Involvement in disease
Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
Sequence similarities
Belongs to the IL-4/IL-13 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3565 Human
- Omim: 147780 Human
- SwissProt: P05112 Human
- Unigene: 73917 Human
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Alternative names
- B cell growth factor 1 antibody
- B cell IgG differentiation factor antibody
- B Cell Stimulatory Factor 1 antibody
see all
Images
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Flow Cytometry - Anti-IL-4 antibody [EPR1118Y] (ab62351)
Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling IL4 with unpurified ab62351 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Immunocytochemistry/ Immunofluorescence - Anti-IL-4 antibody [EPR1118Y] (ab62351)](https://www.abcam.com/ps/products/62/ab62351/Images/ab62351-262046-anti-il-4-antibody-epr1118y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL-4 antibody [EPR1118Y] (ab62351)Immunocytochemistry/Immunofluorescence analysis of RAMOS (human Burkitt's lymphoma) labelling IL4 with purified ab62351 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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![Western blot - Anti-IL-4 antibody [EPR1118Y] (ab62351)](https://www.abcam.com/ps/products/62/ab62351/Images/ab62351-75666-anti-il-4-antibody-epr1118y-western-blot.jpg)
Western blot - Anti-IL-4 antibody [EPR1118Y] (ab62351)Anti-IL-4 antibody [EPR1118Y] (ab62351) at 1/2000 dilution + Human tonsil lysate at 10 µg
Secondary
goat anti rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted? -
![Anti-IL-4 antibody [EPR1118Y] (ab62351)](https://www.abcam.com/ps/products/62/ab62351/Images/ab62351-4-benefits-of-recombinant-antibodies.png)
Anti-IL-4 antibody [EPR1118Y] (ab62351)
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab62351 has been referenced in 5 publications.
- Qu S et al. MicroRNA-194 reduces inflammatory response and human dermal microvascular endothelial cells permeability through suppression of TGF-ß/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria. J Cell Biochem 121:111-124 (2020). PubMed: 31190349
- Becares N et al. Immune Regulatory Mediators in Plasma from Patients With Acute Decompensation Are Associated With 3-Month Mortality. Clin Gastroenterol Hepatol 18:1207-1215.e6 (2020). PubMed: 31446184
- Granier C et al. Formation of somatosensory detour circuits mediates functional recovery following dorsal column injury. Sci Rep 10:10953 (2020). PubMed: 32616790
- Tao S et al. Linc00514 promotes breast cancer metastasis and M2 polarization of tumor-associated macrophages via Jagged1-mediated notch signaling pathway. J Exp Clin Cancer Res 39:191 (2020). PubMed: 32943090
- Xie N et al. miR-320a upregulation contributes to the development of preeclampsia by inhibiting the growth and invasion of trophoblast cells by targeting interleukin 4. Mol Med Rep 20:3256-3264 (2019). PubMed: 31432141
