Recombinant
RabMAb

Recombinant Anti-IL-4 antibody [EPR1118Y] - BSA and Azide free (ab246802)

Overview

  • Product name

    Anti-IL-4 antibody [EPR1118Y] - BSA and Azide free
    See all IL-4 primary antibodies
  • Description

    Rabbit monoclonal [EPR1118Y] to IL-4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IL-4 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human tonsil lysate
  • General notes

    ab246802 is the carrier-free version of ab62351 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab246802 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab246802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
    • Involvement in disease

      Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
    • Sequence similarities

      Belongs to the IL-4/IL-13 family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • B cell growth factor 1 antibody
      • B cell IgG differentiation factor antibody
      • B Cell Stimulatory Factor 1 antibody
      • B-cell stimulatory factor 1 antibody
      • BCGF 1 antibody
      • BCGF1 antibody
      • Binetrakin antibody
      • BSF-1 antibody
      • BSF1 antibody
      • IGG1 induction factor antibody
      • IL 4 antibody
      • IL-4 antibody
      • IL4 antibody
      • IL4_HUMAN antibody
      • Il4e12 antibody
      • Interleukin 4 antibody
      • Interleukin 4 variant 2 antibody
      • Interleukin 4, isoform 1 antibody
      • Interleukin-4 antibody
      • Lymphocyte stimulatory factor 1 antibody
      • MGC79402 antibody
      • Pitrakinra antibody
      see all

    Images

    • Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling IL4 with unpurified ab62351 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62351).

    • Immunocytochemistry/Immunofluorescence analysis of RAMOS (human Burkitt's lymphoma) labelling IL4 with purified ab62351 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Control: PBS only

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62351).

    References

    ab246802 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab246802.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up