Recombinant
RabMAb

Recombinant Anti-IL-4 antibody [EPR22879-236] (ab225638)

Overview

  • Product name

    Anti-IL-4 antibody [EPR22879-236]
    See all IL-4 primary antibodies
  • Description

    Rabbit monoclonal [EPR22879-236] to IL-4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P,IP or WB
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse IL-4 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P07750

  • Positive control

    • FC: Mouse splenocytes treated with 2.5µg/ml concanavalin A for 3 days.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P,IP or WB.
  • Target

    • Function

      Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
    • Involvement in disease

      Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
    • Sequence similarities

      Belongs to the IL-4/IL-13 family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • B cell growth factor 1 antibody
      • B cell IgG differentiation factor antibody
      • B Cell Stimulatory Factor 1 antibody
      • B-cell stimulatory factor 1 antibody
      • BCGF 1 antibody
      • BCGF1 antibody
      • Binetrakin antibody
      • BSF-1 antibody
      • BSF1 antibody
      • IGG1 induction factor antibody
      • IL 4 antibody
      • IL-4 antibody
      • IL4 antibody
      • IL4_HUMAN antibody
      • Il4e12 antibody
      • Interleukin 4 antibody
      • Interleukin 4 variant 2 antibody
      • Interleukin 4, isoform 1 antibody
      • Interleukin-4 antibody
      • Lymphocyte stimulatory factor 1 antibody
      • MGC79402 antibody
      • Pitrakinra antibody
      see all

    Images

    • Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse splenocytes treated with 2.5μg/ml concanavalin A for 3 days cells labelling IL-4 with ab225638 at 1/50 dilution.

      A Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/2000 dilution was used as the secondary antibody. 

      Cells were surface stained with anti-CD4 conjugated to APC/Fire™ 750, then fixed with 2% PFA for 10min followed by intracellularly stained with anti-IL4 conjugated to PE/Cy7 and rabbit IgG (Left) or ab225248 (Right).
      Gated on CD4+T cells.

    References

    ab225638 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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