Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22879-236] to IL-4
- Suitable for: Flow Cyt
- Reacts with: Mouse
Product nameAnti-IL-4 antibody [EPR22879-236]
See all IL-4 primary antibodies
DescriptionRabbit monoclonal [EPR22879-236] to IL-4
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse IL-4 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P07750
- Flow Cyt: Mouse splenocytes treated with 2.5g/ml concanavalin A for 3 days.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab225638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionParticipates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
Involvement in diseaseGenetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Sequence similaritiesBelongs to the IL-4/IL-13 family.
- Information by UniProt
- B cell growth factor 1 antibody
- B cell IgG differentiation factor antibody
- B Cell Stimulatory Factor 1 antibody
Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse splenocytes treated with 2.5μg/ml concanavalin A for 3 days cells labelling IL-4 with ab225638 at 1/50 dilution.
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with anti-CD4 conjugated to APC/Fire™ 750, then fixed with 2% PFA for 10min followed by intracellularly stained with anti-IL4 conjugated to PE/Cy7 and rabbit IgG (Left) or ab225248 (Right).
Gated on CD4+T cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225638 has not yet been referenced specifically in any publications.