Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22879-236] to IL-4 - BSA and Azide free
- Suitable for: Flow Cyt (Intra)
- Reacts with: Mouse
Related conjugates and formulations
Product nameAnti-IL-4 antibody [EPR22879-236] - BSA and Azide free
See all IL-4 primary antibodies
DescriptionRabbit monoclonal [EPR22879-236] to IL-4 - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt (Intra)more details
Unsuitable for: ICC/IF,IHC-P,IP or WB
Species reactivityReacts with: Mouse
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
- Flow Cyt (intra): Mouse splenocytes treated with 2.5g/ml concanavalin A for 3 days.
ab261917 is the carrier-free version of ab225638.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab261917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt (Intra)||
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
FunctionParticipates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
Involvement in diseaseGenetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Sequence similaritiesBelongs to the IL-4/IL-13 family.
- Information by UniProt
- B cell growth factor 1 antibody
- B cell IgG differentiation factor antibody
- B Cell Stimulatory Factor 1 antibody
Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse splenocytes treated with 2.5μg/ml concanavalin A for 3 days cells labelling IL-4 with ab225638 at 1/50 dilution.
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with anti-CD4 conjugated to APC/Fire™ 750, then fixed with 2% PFA for 10min followed by intracellularly stained with anti-IL4 conjugated to PE/Cy7 and rabbit IgG (Left) or ab225248 (Right).
Gated on CD4+T cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225638).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab261917 has not yet been referenced specifically in any publications.