Recombinant Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)](https://www.abcam.com/ps/products/222/ab222102/Images/ab222102-315243-anti-il-4i1-lao-antibody-epr22070-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22070] to IL-4I1/LAO
- Suitable for: IP, WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-IL-4I1/LAO antibody [EPR22070]
See all IL-4I1/LAO primary antibodies -
Description
Rabbit monoclonal [EPR22070] to IL-4I1/LAO -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag, whole cell lysate . IHC-P: Human tonsil, Hodgkin's lymphoma and diffuse large B-cell lymphoma tissue. ICC/IF: HEK-293 whole cell lysate. Flow: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag. IP: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22070 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| IP |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/30.
|
|
| WB |
1/1000. Predicted molecular weight: 63 kDa.
|
|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| Flow Cyt |
1/600.
|
|
| ICC/IF |
1/50.
|
| Notes |
|---|
|
IP
1/30. |
|
WB
1/1000. Predicted molecular weight: 63 kDa. |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
Flow Cyt
1/600. |
|
ICC/IF
1/50. |
Target
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Relevance
IL-4-induced gene-1 (Il-4i1 or Fig1) is expressed in primarily immune tissues and genetically maps to a region of susceptibility to autoimmune disease. The predicted Il-4i1 protein (IL-4I1) sequence is most similar to apoptosis-inducing protein and Apoxin I, which are both l-amino acid oxidases. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 259307 Human
- Omim: 609742 Human
- SwissProt: Q1WMJ3 Human
- SwissProt: Q96RQ9 Human
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Alternative names
- FIG 1 antibody
- Fig 1 protein antibody
- FIG1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
Immunohistochemical analysis of paraffin-embedded human difuse large B-cell lymphoma tissue stained for IL-4I1/LAO with ab222102 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Granular cytoplasmic staining in human diffuse large B-cell lymphoma (PMID: 19436310) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)](https://www.abcam.com/ps/products/222/ab222102/Images/ab222102-315242-anti-il-4i1-lao-antibody-epr22070-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue stained for IL-4I1/LAO with ab222102 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Granular cytoplasmic staining in human Hodgkin's lymphoma (PMID: 19436310) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)](https://www.abcam.com/ps/products/222/ab222102/Images/ab222102-315241-anti-il-4i1-lao-antibody-epr22070-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)Immunohistochemical analysis of paraffin-embedded human tonsil tissue stained for IL-4I1/LAO with ab222102 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Granular cytoplasmic staining in macrophages of human tonsil (PMID: 17356132; PMID: 19436310) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)](https://www.abcam.com/ps/products/222/ab222102/Images/ab222102-315240-anti-il-4i1-lao-antibody-epr22070-immunoprecipitation.jpg)
Immunoprecipitation - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)IL-4I1/LAO was immunoprecipitated from 0.35mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate with ab222102 at 1/30 dilution. Western blot was perfromed from the immunoprecipitate using ab222102 at 1/1000 dilution, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate 10μg (Input).
Lane 2: ab222102 IP in HEK-293 transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222102 in HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate (-).Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 seconds. -
![Flow Cytometry - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)](https://www.abcam.com/ps/products/222/ab222102/Images/ab222102-315239-anti-il-4i1-lao-antibody-epr22070-flow-cytometry.jpg)
Flow Cytometry - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag labeling IL-4I1/LAO with ab222102 at 1/500 dilution (Right) compared to Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)](https://www.abcam.com/ps/products/222/ab222102/Images/ab222102-315238-anti-il-4i1-lao-antibody-epr22070-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilzed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling IL-4I1/LAO (white) with ab222102 at 1/50 dilution, followed by ab150079 AlexaFluor®647 Goat anti-Rabbit secondary at 1/1000 dilution. Confocal image showing cytoplasmic staining in HEK-293T cells transfected with GFP-tagged IL-4I1/LAO expression vector. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150079 AlexaFluor®647 Goat anti-Rabbit secondary at 1/1000 dilution.
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![Western blot - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)](https://www.abcam.com/ps/products/222/ab222102/Images/ab222102-315237-anti-il-4i1-lao-antibody-epr22070-western-blot.jpg)
Western blot - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)All lanes : Anti-IL-4I1/LAO antibody [EPR22070] (ab222102) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-GFP-tag, whole cell lysate
Lane 2 : HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 63 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 6 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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![Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)](https://www.abcam.com/ps/products/222/ab222102/Images/ab222102-8-benefits-of-recombinant-antibodies.png)
Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab222102 has not yet been referenced specifically in any publications.
