Recombinant
RabMAb

Recombinant Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

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Overview

  • Product name

    Anti-IL-4I1/LAO antibody [EPR22070]
    See all IL-4I1/LAO primary antibodies

  • Description

    Rabbit monoclonal [EPR22070] to IL-4I1/LAO

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human IL-4I1/LAO aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96RQ9

  • Positive control

    • WB: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag, whole cell lysate . IHC-P: Human tonsil, Hodgkin's lymphoma and diffuse large B-cell lymphoma tissue. ICC/IF: HEK-293 whole cell lysate. Flow: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag. IP: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22070

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab222102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
WB 1/1000. Predicted molecular weight: 63 kDa.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/600.
ICC/IF 1/50.

Target

  • Relevance

    IL-4-induced gene-1 (Il-4i1 or Fig1) is expressed in primarily immune tissues and genetically maps to a region of susceptibility to autoimmune disease. The predicted Il-4i1 protein (IL-4I1) sequence is most similar to apoptosis-inducing protein and Apoxin I, which are both l-amino acid oxidases.

  • Cellular localization

    Cytoplasmic

  • Database links

    • Alternative names

      • FIG 1 antibody
      • Fig 1 protein antibody
      • FIG1 antibody
      • Fig1 protein antibody
      • HCG2043070 antibody
      • hFIG 1 antibody
      • hFIG1 antibody
      • IL4 induced protein 1 antibody
      • IL4I1 antibody
      • Interleukin 4 induced 1 antibody
      • Interleukin 4 induced protein 1 antibody
      • Interleukin four induced 1 antibody
      • L amino acid oxidase antibody
      • LAAO antibody
      • LAO antibody
      • Protein Fig 1 antibody
      • Protein Fig1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

      Immunohistochemical analysis of paraffin-embedded human difuse large B-cell lymphoma tissue stained for IL-4I1/LAO with ab222102 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Granular cytoplasmic staining in human diffuse large B-cell lymphoma (PMID: 19436310) is observed. Counterstained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

      Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue stained for IL-4I1/LAO with ab222102 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Granular cytoplasmic staining in human Hodgkin's lymphoma (PMID: 19436310) is observed. Counterstained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue stained for IL-4I1/LAO with ab222102 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Granular cytoplasmic staining in macrophages of human tonsil (PMID: 17356132; PMID: 19436310) is observed. Counterstained with Hematoxylin.


      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    • Immunoprecipitation - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      Immunoprecipitation - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

      IL-4I1/LAO was immunoprecipitated from 0.35mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate with ab222102 at 1/30 dilution. Western blot was perfromed from the immunoprecipitate using ab222102 at 1/1000 dilution, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

      Lane 1: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate 10μg (Input).

      Lane 2: ab222102 IP in HEK-293 transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate (+).

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222102 in HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate (-).

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 1 seconds.

    • Flow Cytometry - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      Flow Cytometry - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag labeling IL-4I1/LAO with ab222102 at 1/500 dilution (Right) compared to Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      Immunocytochemistry/ Immunofluorescence - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilzed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling IL-4I1/LAO (white) with ab222102 at 1/50 dilution, followed by ab150079 AlexaFluor®647 Goat anti-Rabbit secondary at 1/1000 dilution. Confocal image showing cytoplasmic staining in HEK-293T cells transfected with GFP-tagged IL-4I1/LAO expression vector. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150079 AlexaFluor®647 Goat anti-Rabbit secondary at 1/1000 dilution.

    • Western blot - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      Western blot - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      All lanes : Anti-IL-4I1/LAO antibody [EPR22070] (ab222102) at 1/1000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-GFP-tag, whole cell lysate
      Lane 2 : HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 63 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?


      Exposure time: 6 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

    References

    ab222102 has not yet been referenced specifically in any publications.

    Publishing research using ab222102? Please let us know so that we can cite the reference in this datasheet.

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