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Cell Biology Apoptosis Intracellular Associated Proteins
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RecombinantRabMAb

Recombinant Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

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  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
  • Immunoprecipitation - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
  • Flow Cytometry - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
  • Western blot - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
  • Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22070] to IL-4I1/LAO
  • Suitable for: IP, WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-IL-4I1/LAO antibody [EPR22070]
    See all IL-4I1/LAO primary antibodies
  • Description

    Rabbit monoclonal [EPR22070] to IL-4I1/LAO
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag, whole cell lysate . IHC-P: Human tonsil, Hodgkin's lymphoma and diffuse large B-cell lymphoma tissue. ICC/IF: HEK-293 whole cell lysate. Flow: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag. IP: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22070
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins

Associated products

  • Alternative Versions

    • Anti-IL-4I1/LAO antibody [EPR22070] - BSA and Azide free (ab237783)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab222102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
IP
Human
WB
Human
Application Abreviews Notes
IP
1/30.
WB
1/1000. Predicted molecular weight: 63 kDa.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
1/600.
ICC/IF
1/50.
Notes
IP
1/30.
WB
1/1000. Predicted molecular weight: 63 kDa.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
1/600.
ICC/IF
1/50.

Target

  • Relevance

    IL-4-induced gene-1 (Il-4i1 or Fig1) is expressed in primarily immune tissues and genetically maps to a region of susceptibility to autoimmune disease. The predicted Il-4i1 protein (IL-4I1) sequence is most similar to apoptosis-inducing protein and Apoxin I, which are both l-amino acid oxidases.
  • Cellular localization

    Cytoplasmic
  • Database links

    • Entrez Gene: 259307 Human
    • Omim: 609742 Human
    • SwissProt: Q1WMJ3 Human
    • SwissProt: Q96RQ9 Human
    • Alternative names

      • FIG 1 antibody
      • Fig 1 protein antibody
      • FIG1 antibody
      • Fig1 protein antibody
      • HCG2043070 antibody
      • hFIG 1 antibody
      • hFIG1 antibody
      • IL4 induced protein 1 antibody
      • IL4I1 antibody
      • Interleukin 4 induced 1 antibody
      • Interleukin 4 induced protein 1 antibody
      • Interleukin four induced 1 antibody
      • L amino acid oxidase antibody
      • LAAO antibody
      • LAO antibody
      • Protein Fig 1 antibody
      • Protein Fig1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

      Immunohistochemical analysis of paraffin-embedded human difuse large B-cell lymphoma tissue stained for IL-4I1/LAO with ab222102 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Granular cytoplasmic staining in human diffuse large B-cell lymphoma (PMID: 19436310) is observed. Counterstained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

      Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue stained for IL-4I1/LAO with ab222102 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Granular cytoplasmic staining in human Hodgkin's lymphoma (PMID: 19436310) is observed. Counterstained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue stained for IL-4I1/LAO with ab222102 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Granular cytoplasmic staining in macrophages of human tonsil (PMID: 17356132; PMID: 19436310) is observed. Counterstained with Hematoxylin.


      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    • Immunoprecipitation - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      Immunoprecipitation - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

      IL-4I1/LAO was immunoprecipitated from 0.35mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate with ab222102 at 1/30 dilution. Western blot was perfromed from the immunoprecipitate using ab222102 at 1/1000 dilution, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

      Lane 1: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate 10μg (Input).

      Lane 2: ab222102 IP in HEK-293 transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate (+).

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222102 in HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate (-).

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 1 seconds.

    • Flow Cytometry - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      Flow Cytometry - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag labeling IL-4I1/LAO with ab222102 at 1/500 dilution (Right) compared to Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      Immunocytochemistry/ Immunofluorescence - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilzed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling IL-4I1/LAO (white) with ab222102 at 1/50 dilution, followed by ab150079 AlexaFluor®647 Goat anti-Rabbit secondary at 1/1000 dilution. Confocal image showing cytoplasmic staining in HEK-293T cells transfected with GFP-tagged IL-4I1/LAO expression vector. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150079 AlexaFluor®647 Goat anti-Rabbit secondary at 1/1000 dilution.

    • Western blot - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      Western blot - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      All lanes : Anti-IL-4I1/LAO antibody [EPR22070] (ab222102) at 1/1000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-GFP-tag, whole cell lysate
      Lane 2 : HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 63 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?


      Exposure time: 6 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)
      Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab222102? Please let us know so that we can cite the reference in this datasheet.

    ab222102 has not yet been referenced specifically in any publications.

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