Key features and details
- Rabbit polyclonal to IL-4R
- Suitable for: ICC/IF, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IL-4R antibody
See all IL-4R primary antibodies
DescriptionRabbit polyclonal to IL-4R
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human colon carcinoma tissue. ICC/IF: A431 cells Flow Cyt: Raji cells
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA
Aqueous buffered solution
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||1/20 - 1/100.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/100 - 1/500.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200.
FunctionReceptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammmation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
Tissue specificityIsoform 1 and isoform 2 are highly expressed in activated T-cells.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain.
DomainThe extracellular domain represents the IL4 binding protein (IL4BP).
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
modificationsOn IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.
The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD124 antibody
- IL 4R alpha antibody
- IL-4 receptor subunit alpha antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling IL4R with ab203398 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Flow Cytometry analysis of Raji cells labeling IL-4R with ab203398. Cells were fixed with 2% PFA (10min at room temperature). The cells were then incubated in 1 X PBS containing 0.5% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Staining with ab203398 was performed at 1/100 for 30 min at room temperature. The secondary antibody, Goat anti-rabbit IgG-PE, was used at a 1/200 dilution for 40 min at room temperature. Primary antibody staining (green) is compared to compared to unstained cells (dark blue), secondary only (light blue), and isotype control (orange).
Immunocytochemistry/ Immunofluorescence analys of A431 cells labeling IL-4R with ab203398 1/100 for 90 minutes at 37°C. Cells were fixed with 4% Paraformaldehyde, Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min. Secondary antibody was Goat Anti-Rabbit IgG antibody with FITC conjugation at 37°C for 90 minutes, DAPI (5ug/ml, blue) was used to stain the cell nuclei.
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for IL4R using ab203398 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, AF488 conjugated at 1/200 dilution.
ab203398 has not yet been referenced specifically in any publications.