Product nameAnti-IL-4R (phospho Y497) antibody
See all IL-4R primary antibodies
DescriptionRabbit polyclonal to IL-4R (phospho Y497)
SpecificityDetects endogenous levels of IL4R only when phosphorylated at tyrosine 497 (corresponding to mouse tyrosine 500)
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human IL-4R.
- 293 and HeLa cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from rabbit antiserum by affinity chromatography, using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab61099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).|
|ICC/IF||1/500 - 1/1000.|
FunctionReceptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammmation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
Tissue specificityIsoform 1 and isoform 2 are highly expressed in activated T-cells.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain.
DomainThe extracellular domain represents the IL4 binding protein (IL4BP).
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
modificationsOn IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.
The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD124 antibody
- IL 4R alpha antibody
- IL-4 receptor subunit alpha antibody
All lanes : Anti-IL-4R (phospho Y497) antibody (ab61099) at 1/500 dilution
Lane 1 : Extracts from 293 cells
Lane 2 : Extracts from 293 cells with immunizing phosphopeptide
Predicted band size: 89 kDa
Observed band size: 89 kDa
ICC/IF image of ab61099 (at a 1/500 dilution) stained HeLa cells, minus immunising peptide (left image) and plus immunising peptide (right image).
ab61099 has been referenced in 1 publication.
- Kurgonaite K et al. Essential role of endocytosis for interleukin-4-receptor-mediated JAK/STAT signalling. J Cell Sci 128:3781-95 (2015). PubMed: 26306492