Anti-IL-5 antibody (ab9624)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-IL-5 antibody
See all IL-5 primary antibodies -
Description
Rabbit polyclonal to IL-5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISA, Neutralisingmore details -
Species reactivity
Reacts with: Human -
Immunogen
Highly pure (>98%) recombinant hIL-5 (human Interleukin-5)
Properties
-
Form
Lyophilised:Reconstitute with 200µl of sterile water. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
PBS, pH 7.4, no preservative, sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab9624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ELISA | ||
| Neutralising |
Application notes
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hIL-5 (0.45 ng/ml), a concentration of 0.05 - 0.09 µg/ml of this antibody is required.
ELISA:To detect hIL-5 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-5.
Western Blot:To detect hIL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA:To detect hIL-5 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-5.
Western Blot:To detect hIL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Target
-
Function
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. -
Sequence similarities
Belongs to the IL-5 family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 3567 Human
- Omim: 147850 Human
- SwissProt: P05113 Human
- Unigene: 2247 Human
-
Alternative names
- B-cell differentiation factor I antibody
- Colony stimulating factor antibody
- EDF antibody
see all
Protocols
Datasheets and documents
References
ab9624 has not yet been referenced specifically in any publications.
