Anti-IL-5 antibody (ab9624)
- Datasheet
- References
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Overview
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Product nameAnti-IL-5 antibody
See all IL-5 primary antibodies -
DescriptionRabbit polyclonal to IL-5
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ELISA, Neutralisingmore details
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Species reactivityReacts with: Human
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Immunogen
Highly pure (>98%) recombinant hIL-5 (human Interleukin-5)
Properties
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FormLyophilised:Reconstitute with 200µl of sterile water.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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Isotypeunknown
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab9624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ELISA | ||
| Neutralising |
Application notes
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hIL-5 (0.45 ng/ml), a concentration of 0.05 - 0.09 µg/ml of this antibody is required.
ELISA:To detect hIL-5 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-5.
Western Blot:To detect hIL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA:To detect hIL-5 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-5.
Western Blot:To detect hIL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Target
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FunctionFactor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
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Sequence similaritiesBelongs to the IL-5 family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 3567 Human
- Omim: 147850 Human
- SwissProt: P05113 Human
- Unigene: 2247 Human
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Alternative names
- B-cell differentiation factor I antibody
- Colony stimulating factor antibody
- EDF antibody
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Datasheets and documents
Protocols
References
ab9624 has not yet been referenced specifically in any publications.
