Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CAL40] to IL-5RA
- Suitable for: ELISA, IHC-P
- Reacts with: Human
Product nameAnti-IL-5RA antibody [CAL40]
See all IL-5RA primary antibodies
DescriptionRabbit monoclonal [CAL40] to IL-5RA
Tested applicationsSuitable for: ELISA, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF,IP or WB
Species reactivityReacts with: Human
Synthetic peptide within Human IL-5RA aa 21-342. The exact sequence is proprietary.
DLLPDEKISLLPPVNFTIKVTGLAQVLLQWKPNPDQEQRNVNLEYQVKIN APKEDDYETRITESKCVTILHKGFSASVRTILQNDHSLLASSWASAELHA PPGSPGTSIVNLTCTTNTTEDNYSRLRSYQVSLHCTWLVGTDAPEDTQYF LYYRYGSWTEECQEYSKDTLGRNIACWFPRTFILSKGRDWLAVLVNGSSK HSAIRPFDQLFALHAIDQINPPLNVTAEIEGTRLSIQWEKPVSAFPIHCF DYEVKIHNTRNGYLQIEKLMTNAFISIIDDLSKYDVQVRAAVSSMCRE AGLWSEWSQPIYVGNDEHKPLREW
Database link: Q01344
- IHC-P: Human tonsil tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesPurity >99%.
Our Abpromise guarantee covers the use of ab237716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC-P||1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThis is the receptor for interleukin-5. The alpha chain binds to IL5.
Tissue specificityExpressed on eosinophils and basophils.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 5 subfamily.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
- Information by UniProt
- CD125 antibody
- CD125 antigen antibody
- CDw125 antibody
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-5RA with ab237716 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human tonsil is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
The section was incubated with ab237716 for 15 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for IL5RA using ab237716 at 0.125 μg/ml in immunohistochemical analysis.
ELISA analysis of Human IL5RA recombinant protein at 1000 ng/mL with ab237716. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237716 has not yet been referenced specifically in any publications.