Recombinant Anti-IL-5RA antibody [EPR5450(2)] (ab134935)
![Western blot - Anti-IL-5RA antibody [EPR5450(2)] (ab134935)](https://www.abcam.com/ps/products/134/ab134935/Images/ab134935-149120-anti-il-5ra-antibody-epr54502-western-blot.jpg "Western blot - Anti-IL-5RA antibody [EPR5450(2)] (ab134935)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5450(2)] to IL-5RA
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-IL-5RA antibody [EPR5450(2)]
See all IL-5RA primary antibodies -
Description
Rabbit monoclonal [EPR5450(2)] to IL-5RA -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human IL-5RA aa 350-450 (C terminal). The exact sequence is proprietary.
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Positive control
- Human platelet and serum lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5450(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
Target
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Function
This is the receptor for interleukin-5. The alpha chain binds to IL5. -
Tissue specificity
Expressed on eosinophils and basophils. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 5 subfamily. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3568 Human
- Omim: 147851 Human
- SwissProt: Q01344 Human
- Unigene: 207105 Human
- Unigene: 68876 Human
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Alternative names
- CD125 antibody
- CD125 antigen antibody
- CDw125 antibody
see all
Images
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Western blot - Anti-IL-5RA antibody [EPR5450(2)] (ab134935)All lanes : Anti-IL-5RA antibody [EPR5450(2)] (ab134935) at 1/1000 dilution
Lane 1 : Human platelet lysate
Lane 2 : Human serum lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 48 kDa -
![Anti-IL-5RA antibody [EPR5450(2)] (ab134935)](https://www.abcam.com/ps/products/134/ab134935/Images/ab134935-2-benefits-of-recombinant-antibodies.png)
Anti-IL-5RA antibody [EPR5450(2)] (ab134935)
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab134935 has not yet been referenced specifically in any publications.