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Immunology Innate Immunity Cytokines Interleukins
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RecombinantRabMAb

Recombinant Anti-IL-5RA antibody [EPR5450(2)] (ab134935)

  • Datasheet
  • SDS
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Western blot - Anti-IL-5RA antibody [EPR5450(2)] (ab134935)
  • Anti-IL-5RA antibody [EPR5450(2)] (ab134935)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5450(2)] to IL-5RA
  • Suitable for: WB
  • Reacts with: Human

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Recombinant Human IL-5RA protein (ab221341)

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Overview

  • Product name

    Anti-IL-5RA antibody [EPR5450(2)]
    See all IL-5RA primary antibodies
  • Description

    Rabbit monoclonal [EPR5450(2)] to IL-5RA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IL-5RA aa 350-450 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human platelet and serum lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5450(2)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins

Associated products

  • Alternative Versions

    • Anti-IL-5RA antibody [EPR5450(2)] - BSA and Azide free (ab248740)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human IL-5RA protein (ab221341)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      This is the receptor for interleukin-5. The alpha chain binds to IL5.
    • Tissue specificity

      Expressed on eosinophils and basophils.
    • Sequence similarities

      Belongs to the type I cytokine receptor family. Type 5 subfamily.
    • Domain

      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q01344 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3568 Human
      • Omim: 147851 Human
      • SwissProt: Q01344 Human
      • Unigene: 207105 Human
      • Unigene: 68876 Human
      • Alternative names

        • CD125 antibody
        • CD125 antigen antibody
        • CDw125 antibody
        • HSIL5R3 antibody
        • IL-5 receptor subunit alpha antibody
        • IL-5R subunit alpha antibody
        • IL-5R-alpha antibody
        • IL-5RA antibody
        • IL5R antibody
        • IL5RA antibody
        • IL5RA_HUMAN antibody
        • Interleukin 5 Receptor alpha antibody
        • Interleukin 5 Receptor alpha chain antibody
        • Interleukin 5 receptor alpha subunit antibody
        • Interleukin 5 receptor subunit alpha antibody
        • Interleukin 5 receptor type 3 antibody
        • Interleukin-5 receptor subunit alpha antibody
        • MGC26560 antibody
        see all

      Images

      • Western blot - Anti-IL-5RA antibody [EPR5450(2)] (ab134935)
        Western blot - Anti-IL-5RA antibody [EPR5450(2)] (ab134935)
        All lanes : Anti-IL-5RA antibody [EPR5450(2)] (ab134935) at 1/1000 dilution

        Lane 1 : Human platelet lysate
        Lane 2 : Human serum lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 48 kDa

      • Anti-IL-5RA antibody [EPR5450(2)] (ab134935)
        Anti-IL-5RA antibody [EPR5450(2)] (ab134935)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab134935? Please let us know so that we can cite the reference in this datasheet.

      ab134935 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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