Overview

  • Product name
  • Description
    Rabbit polyclonal to IL-6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Functional Studies, ELISA, IRMA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse

Properties

Applications

Our Abpromise guarantee covers the use of ab7737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Dilutions have to be made according to the amount of IL-6 to be inactivated.

The antibody can be used for neutralization of mouse and rat IL-6 in bio-assays. For neutralization of biological activity dilutions have to be made according to the amounts IL-6 to be inactivated.

ELISA Use at an assay dependent concentration.
IRMA Use at an assay dependent concentration.
WB 1/20.
IHC-P Use at an assay dependent concentration. PubMed: 24763697
ICC/IF Use at an assay dependent concentration. PubMed: 24763697

Target

  • Function
    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease
    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Interleukin BSF 2 antibody
    • B cell differentiation factor antibody
    • B cell stimulatory factor 2 antibody
    • B-cell stimulatory factor 2 antibody
    • BSF 2 antibody
    • BSF-2 antibody
    • BSF2 antibody
    • CDF antibody
    • CTL differentiation factor antibody
    • Hepatocyte stimulatory factor antibody
    • HGF antibody
    • HSF antibody
    • Hybridoma growth factor antibody
    • Hybridoma growth factor Interferon beta-2 antibody
    • IFN-beta-2 antibody
    • IFNB2 antibody
    • IL 6 antibody
    • IL-6 antibody
    • IL6 antibody
    • IL6_HUMAN antibody
    • Interferon beta 2 antibody
    • Interferon beta-2 antibody
    • Interleukin 6 antibody
    • Interleukin 6 (interferon beta 2) antibody
    • Interleukin BSF 2 antibody
    • Interleukin-6 antibody
    see all

References

This product has been referenced in:
  • Park JS  et al. A probiotic complex, rosavin, zinc, and prebiotics ameliorate intestinal inflammation in an acute colitis mouse model. J Transl Med 16:37 (2018). Read more (PubMed: 29466999) »
  • Cao W  et al. Homeobox a5 Promotes White Adipose Tissue Browning Through Inhibition of the Tenascin C/Toll-Like Receptor 4/Nuclear Factor Kappa B Inflammatory Signaling in Mice. Front Immunol 9:647 (2018). Read more (PubMed: 29651293) »
See all 14 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab7737 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 120 days of purchase), and if it appears that the antibody is at fault, a replacement/credit note/refund will be offered. After looking at the protocols you used, I am surprised that you did not manage to obtain any bands even with 160ug of protein. However, it is very hard for me to determine the cause without more details of your WB protocol. If you would be so kind to clarify the following items, it will certainly help me better understand the root of the cause and as a result, I will be able to tag this product as faulty. - what type of cells are you are using? Are they known to have sufficient levels for detection? - could the no signal you are experiencing be due to poor transfer of protein to membrane? Did you check the transfer efficiency? This can be simply done with a reversible stain such as Ponceau S. - At Abcam, almost all of our products has been tested with 5% BSA. Sometimes, a different blocking buffer might cause cross reaction between the blocking agent and antibodies, therefore causing no bands to appear. I would recommend you try 5% BSA, if you have not already done so. - can you confirm that the second antibody you used also managed to produce good results with other primary antibodies? Regarding the recommended starting dilution, there is a note on the datasheet that states “Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Dilutions have to be made according to the amount of IL-6 to be inactivated. For Western blots the material should be diluted 20 times.” I am sorry for the string of questions but I hope the above suggestions may already help you. If you have already tried the above suggestions and still experience problems, please do not hesitate to contact me with details of your order (purchase order number, batch number, contact information, shipping address/purchasing agent, etc.). Also, please advice on how you would like to proceed with your enquiry, so that I can immediately arrange for a replacement or refund for you. I look forward to hearing from you again in order to resolve the matter.

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Answer

We usually use Nunc Covalink plates, which are available in the UK from Fisher.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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