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    il-6-antibody-ab7737.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Anti-IL-6 antibody (ab7737)

  • Datasheet
Reviews (1)Q&A (2)References (47)

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Key features and details

  • Rabbit polyclonal to IL-6
  • Reacts with: Mouse
  • Isotype: IgG

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Protein
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Recombinant human IL-6 protein (Active) (ab259381)

View more associated products

Overview

  • Product name

    Anti-IL-6 antibody
    See all IL-6 primary antibodies
  • Description

    Rabbit polyclonal to IL-6
  • Host species

    Rabbit
  • Species reactivity

    Reacts with: Mouse

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Neuroscience
    • Neurology process
    • Metabolism
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Metabolic disorders

Associated products

  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human IL-6 protein (Active) (ab259381)

Target

  • Function

    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease

    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P05231 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 16193 Mouse
    • SwissProt: P08505 Mouse
    • Unigene: 1019 Mouse
    • Alternative names

      • Interleukin BSF 2 antibody
      • B cell differentiation factor antibody
      • B cell stimulatory factor 2 antibody
      • B-cell stimulatory factor 2 antibody
      • BSF 2 antibody
      • BSF-2 antibody
      • BSF2 antibody
      • CDF antibody
      • CTL differentiation factor antibody
      • Cytotoxic T cell differentiation factor antibody
      • Hepatocyte stimulating factor antibody
      • Hepatocyte stimulatory factor antibody
      • HGF antibody
      • HSF antibody
      • Hybridoma growth factor antibody
      • Hybridoma growth factor Interferon beta-2 antibody
      • Hybridoma plasmacytoma growth factor antibody
      • IFN-beta-2 antibody
      • IFNB2 antibody
      • IL 6 antibody
      • IL-6 antibody
      • IL6 antibody
      • IL6_HUMAN antibody
      • Interferon beta 2 antibody
      • Interferon beta-2 antibody
      • Interleukin 6 antibody
      • Interleukin 6 (interferon beta 2) antibody
      • Interleukin BSF 2 antibody
      • Interleukin-6 antibody
      see all

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (47)

    Publishing research using ab7737? Please let us know so that we can cite the reference in this datasheet.

    ab7737 has been referenced in 47 publications.

    • Kim SR  et al. Transplanted senescent renal scattered tubular-like cells induce injury in the mouse kidney. Am J Physiol Renal Physiol 318:F1167-F1176 (2020). PubMed: 32223312
    • Xu X  et al. Silencing of NONO inhibits abdominal aortic aneurysm in apolipoprotein E-knockout mice via collagen deposition and inflammatory inhibition. J Cell Mol Med 23:7449-7461 (2019). PubMed: 31512366
    • Lian H  et al. Malignant fibrous histiocytoma amplified sequence 1 alleviates inflammation and renal fibrosis in diabetic nephropathy by inhibiting TLR4. Biosci Rep 39:N/A (2019). PubMed: 31696221
    • Li Q  et al. Total Glycosides of Peony Protects Against Inflammatory Bowel Disease by Regulating IL-23/IL-17 Axis and Th17/Treg Balance. Am J Chin Med 47:177-201 (2019). PubMed: 30612460
    • Wang B  et al. Alisol B 23-Acetate Ameliorates Lipopolysaccharide-Induced Cardiac Dysfunction by Suppressing Toll-Like Receptor 4 (TLR4)/NADPH Oxidase 2 (NOX2) Signaling Pathway. Med Sci Monit 25:8472-8481 (2019). PubMed: 31707400
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-IL-6 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Liver)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (15)
    Loading amount
    30 µg
    Treatment
    transfected with NFAT
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 07 2020

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No signal at all SAMPLE Mouse whole cell extract PRIMARY ANTIBODY ab7737 antibody against IL6. 1:200 dilution, overnight at 4oC followed by 4x10 min wash in TBST DETECTION METHOD ECL, supersignal west pico ANTIBODY STORAGE CONDITIONS -20, in aliquots SAMPLE PREPARATION RIPA buffer with protease inhibitors. Add loading buffer and boil 5 mins prior to loading AMOUNT OF PROTEIN LOADED ~160ug ELECTROPHORESIS/GEL CONDITIONS 15% PAGE, tris-glycine buffer TRANSFER AND BLOCKING CONDITIONS Blocking 30 mins, contains nonfat dry milk and NP40 SECONDARY ANTIBODY anti rabbit, santa cruz HRP conjugated. 1:3000, 1 hour foloowed by 4x10 min TBST washes HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1 HAVE YOU RUN A "NO PRIMARY" CONTROL? No ADDITIONAL NOTES The data sheet says that this antibody has been used for WB, but not even a starting range for dilution is provided. CAn you please provide a range?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 08 2007

    Answer

    Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab7737 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 120 days of purchase), and if it appears that the antibody is at fault, a replacement/credit note/refund will be offered. After looking at the protocols you used, I am surprised that you did not manage to obtain any bands even with 160ug of protein. However, it is very hard for me to determine the cause without more details of your WB protocol. If you would be so kind to clarify the following items, it will certainly help me better understand the root of the cause and as a result, I will be able to tag this product as faulty. - what type of cells are you are using? Are they known to have sufficient levels for detection? - could the no signal you are experiencing be due to poor transfer of protein to membrane? Did you check the transfer efficiency? This can be simply done with a reversible stain such as Ponceau S. - At Abcam, almost all of our products has been tested with 5% BSA. Sometimes, a different blocking buffer might cause cross reaction between the blocking agent and antibodies, therefore causing no bands to appear. I would recommend you try 5% BSA, if you have not already done so. - can you confirm that the second antibody you used also managed to produce good results with other primary antibodies? Regarding the recommended starting dilution, there is a note on the datasheet that states “Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Dilutions have to be made according to the amount of IL-6 to be inactivated. For Western blots the material should be diluted 20 times.” I am sorry for the string of questions but I hope the above suggestions may already help you. If you have already tried the above suggestions and still experience problems, please do not hesitate to contact me with details of your order (purchase order number, batch number, contact information, shipping address/purchasing agent, etc.). Also, please advice on how you would like to proceed with your enquiry, so that I can immediately arrange for a replacement or refund for you. I look forward to hearing from you again in order to resolve the matter.

    Read More

    Abcam Scientific Support

    Answered on Jun 08 2007

    Question

    Would you have a specific recomendation or suggestion of the best plates for running elisas. Which brand do you normally use for yu Elisa plates. We had some problems with some plates and we are ready to use some of you mouse il6 rabbit polyclonal to run some il6 elisa asssyas.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 04 2002

    Answer

    We usually use Nunc Covalink plates, which are available in the UK from Fisher.

    Read More

    Abcam Scientific Support

    Answered on Mar 07 2002

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