Anti-IL-6 antibody (ab7737)

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab7737 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 120 days of purchase), and if it appears that the antibody is at fault, a replacement/credit note/refund will be offered. After looking at the protocols you used, I am surprised that you did not manage to obtain any bands even with 160ug of protein. However, it is very hard for me to determine the cause without more details of your WB protocol. If you would be so kind to clarify the following items, it will certainly help me better understand the root of the cause and as a result, I will be able to tag this product as faulty. - what type of cells are you are using? Are they known to have sufficient levels for detection? - could the no signal you are experiencing be due to poor transfer of protein to membrane? Did you check the transfer efficiency? This can be simply done with a reversible stain such as Ponceau S. - At Abcam, almost all of our products has been tested with 5% BSA. Sometimes, a different blocking buffer might cause cross reaction between the blocking agent and antibodies, therefore causing no bands to appear. I would recommend you try 5% BSA, if you have not already done so. - can you confirm that the second antibody you used also managed to produce good results with other primary antibodies? Regarding the recommended starting dilution, there is a note on the datasheet that states “Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Dilutions have to be made according to the amount of IL-6 to be inactivated. For Western blots the material should be diluted 20 times.” I am sorry for the string of questions but I hope the above suggestions may already help you. If you have already tried the above suggestions and still experience problems, please do not hesitate to contact me with details of your order (purchase order number, batch number, contact information, shipping address/purchasing agent, etc.). Also, please advice on how you would like to proceed with your enquiry, so that I can immediately arrange for a replacement or refund for you. I look forward to hearing from you again in order to resolve the matter.

We usually use Nunc Covalink plates, which are available in the UK from Fisher.