Overview

  • Product name
  • Description
    Goat polyclonal to IL-6
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Highly pure (>98%) recombinant rat IL-6 (rat Interleukin-6)

  • Positive control
    • Recombinant rat IL6 protein (ab9771) can be used as a positive control in WB.

Properties

Applications

Our Abpromise guarantee covers the use of ab9770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. To detect rIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect rIL-6 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rIL-6.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of rIL-6 (0.1 ng/ml), a concentration of 0.002 0.005 µg/ml of this antibody is required.

Target

  • Function
    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease
    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Interleukin BSF 2 antibody
    • B cell differentiation factor antibody
    • B cell stimulatory factor 2 antibody
    • B-cell stimulatory factor 2 antibody
    • BSF 2 antibody
    • BSF-2 antibody
    • BSF2 antibody
    • CDF antibody
    • CTL differentiation factor antibody
    • Hepatocyte stimulatory factor antibody
    • HGF antibody
    • HSF antibody
    • Hybridoma growth factor antibody
    • Hybridoma growth factor Interferon beta-2 antibody
    • IFN-beta-2 antibody
    • IFNB2 antibody
    • IL 6 antibody
    • IL-6 antibody
    • IL6 antibody
    • IL6_HUMAN antibody
    • Interferon beta 2 antibody
    • Interferon beta-2 antibody
    • Interleukin 6 antibody
    • Interleukin 6 (interferon beta 2) antibody
    • Interleukin BSF 2 antibody
    • Interleukin-6 antibody
    see all

Images

  • IL-6-induced collagen synthesis during hypertrophy in vivo and in vitro.
    Serum IL-6 concentration was measured by ELISA on day 15 after renal artery ligation in rats, and results show a significant increase in secreted IL-6 level in the serum of renal artery-ligated (L) animals (721.42 ± 34.45 pg/ml) compared to sham-operated controls (39.33 ± 12.96 pg/ml), which was reduced significantly (48.1%) with neutralizing IL-6 antibody (L+N-IL-6Ab)(ab9770) treatment (10 µg/animal; on alternate days after renal artery ligation till sacrifice).

    Results are expressed as the ±S.E. of three independent experiments. **, p < 0.01 with respect to Sham; ††, p < 0.01 with respect to ligated; n = 10 in each group.

References

This product has been referenced in:
  • Zhou R  et al. Activation of spinal dorsal horn P2Y13 receptors can promote the expression of IL-1ß and IL-6 in rats with diabetic neuropathic pain. J Pain Res 11:615-628 (2018). Read more (PubMed: 29628771) »
  • Mir SA  et al. Inhibition of Signal Transducer and Activator of Transcription 3 (STAT3) Attenuates Interleukin-6 (IL-6)-induced Collagen Synthesis and Resultant Hypertrophy in Rat Heart. J Biol Chem 287:2666-77 (2012). Neutralising ; Rat . Read more (PubMed: 22157761) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry.

As stated on the datasheet, the anti-IL6 antibody ab9770 should be stored at +4ºC.
We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least 6 months from the date of purchase when stored correctly.
General rules suggest that antibodies should be stable for at least a year when stored at +4ºC.

For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols).

I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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