Biotin Anti-IL-6 antibody (ab271236)
Key features and details
- Biotin Goat polyclonal to IL-6
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-IL-6 antibody
See all IL-6 primary antibodies -
Description
Biotin Goat polyclonal to IL-6 -
Host species
Goat -
Conjugation
Biotin -
Tested applications
Suitable for: WB, Sandwich ELISAmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human IL-6 aa 1-150.
Database link: P05231 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 23 kDa.
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Sandwich ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 23 kDa. |
Sandwich ELISA
Use at an assay dependent concentration. |
Target
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Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Interleukin BSF 2 antibody
- B cell differentiation factor antibody
- B cell stimulatory factor 2 antibody
see all
Images
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Western blot analysis of varying levels of Recombinant human IL-6 protein using ab271236 at 0.2 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24 -
Human IL-6 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271236. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human IL-6 protein.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab271236 has been referenced in 2 publications.
- Wikan N et al. The capsaicinoid nonivamide suppresses the inflammatory response and attenuates the progression of steatosis in a NAFLD-rat model. J Biochem Mol Toxicol 37:e23279 (2023). PubMed: 36541345
- Yawoot N et al. Melatonin attenuates reactive astrogliosis and glial scar formation following cerebral ischemia and reperfusion injury mediated by GSK-3β and RIP1K. J Cell Physiol 237:1818-1832 (2022). PubMed: 34825376