Recombinant Anti-IL-6 antibody [EPR21710] - BSA and Azide free (ab229697)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21710] to IL-6 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-IL-6 antibody [EPR21710] - BSA and Azide free
See all IL-6 primary antibodies -
Description
Rabbit monoclonal [EPR21710] to IL-6 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: RAW 264.7 treated with LPS and BFA, whole cell lysate.
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General notes
ab229697 is the carrier-free version of ab229381.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21710 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 16193 Mouse
- SwissProt: P08505 Mouse
- Unigene: 1019 Mouse
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Alternative names
- Interleukin BSF 2 antibody
- B cell differentiation factor antibody
- B cell stimulatory factor 2 antibody
see all
Images
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IL6 was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 100 ng/ml Lipopolysaccharide (LPS) for 6 hours, then added 300 ng/ml BFA the last 3 hours, whole cell lysate with ab229381 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229381 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: RAW 264.7 treated with 100 ng/ml Lipopolysaccharide (LPS) for 6 hours, then added 300 ng/ml BFA the last 3 hours, whole cell lysate 10 µg (Input).
Lane 2: ab229381 IP in RAW 264.7 treated with 100 ng/ml Lipopolysaccharide (LPS) for 6 hours, then added 300 ng/ml BFA the last 3 hours, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229381 in RAW 264.7 treated with 100 ng/ml Lipopolysaccharide (LPS) for 6 hours, then added 300 ng/ml BFA the last 3 hours, whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 2 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229381).
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All lanes : Anti-IL-6 antibody [EPR21710] (ab229381) at 1/1000 dilution
Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus), whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 100 ng/ml Lipopolysaccharides (LPS) for 6 hours, then added 300 ng/ml BFA for the last 3 hours, whole cell lysate, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
We detect a non-specific band at approximately 60 KDa.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229381).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab229697 has not yet been referenced specifically in any publications.