Key features and details
- Mouse monoclonal [F8] to IL-6
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IL-6 antibody [F8]
See all IL-6 primary antibodies
DescriptionMouse monoclonal [F8] to IL-6
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Recombinant fragment corresponding to Rhesus monkey IL-6 aa 27-212. N-terminal His tag. Expressed in E. coli.
PAPVLPGEDSKNVAAPHSQPLTSSERIDKHIRYILDGISALRKETCNRSN MCESSKEALAENNLNLPKMAEKDGCFQSGFNEDTCLVKIITGLLEFEVYL EYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPEPTTNASLL TKLQAQNQWLQDMTTHLILRSFKEFLQSNLRALRQM
Database link: P51494
- IHC-P: Human breast cancer, lung cancer and stomach cancer tissue. WB: Recombinant rhesus monkey IL6 protein. Human leukocyte lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab239482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml.|
FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
Sequence similaritiesBelongs to the IL-6 superfamily.
modificationsN- and O-glycosylated.
- Information by UniProt
- Interleukin BSF 2 antibody
- B cell differentiation factor antibody
- B cell stimulatory factor 2 antibody
Anti-IL-6 antibody [F8] (ab239482) at 2 µg/ml + Human leukocyte lysate
HRP-Goat Anti-Mouse IgG at 0.2 µg/ml
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for IL6 with ab239482 at 30 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for IL6 with ab239482 at 30 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for IL6 with ab239482 at 30 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
Anti-IL-6 antibody [F8] (ab239482) at 2 µg/ml + Recombinant rhesus monkey IL6 protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab239482 has not yet been referenced specifically in any publications.