Key features and details
- Rabbit polyclonal to IL-6 (HRP)
- Suitable for: WB, ELISA
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG
Product nameAnti-IL-6 antibody (HRP)
See all IL-6 primary antibodies
DescriptionRabbit polyclonal to IL-6 (HRP)
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human IL-6.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride, 1% BSA
Do NOT add Sodium Azide!
Concentration information loading...
Purification notesThis purified antibody has been heated to 56°C for 30 minutes.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab106024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 24 kDa. In Western blot analysis of natural cell products or Human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.|
|ELISA||1/10000 - 1/50000.|
FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
Sequence similaritiesBelongs to the IL-6 superfamily.
modificationsN- and O-glycosylated.
- Information by UniProt
- Interleukin BSF 2 antibody
- B cell differentiation factor antibody
- B cell stimulatory factor 2 antibody
Anti-IL-6 antibody (HRP) (ab106024) at 1/1000 dilution (Human peroxidase conjugate) + Human IL-6 at 0.05 µg
Predicted band size: 24 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
ab106024 has not yet been referenced specifically in any publications.