Overview

  • Product name

    Anti-IL-6 antibody (HRP)
    See all IL-6 primary antibodies
  • Description

    Rabbit polyclonal to IL-6 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human IL-6.

Properties

Applications

Our Abpromise guarantee covers the use of ab106024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 24 kDa. In Western blot analysis of natural cell products or Human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.
ELISA 1/10000 - 1/50000.

Target

  • Function

    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease

    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Interleukin BSF 2 antibody
    • B cell differentiation factor antibody
    • B cell stimulatory factor 2 antibody
    • B-cell stimulatory factor 2 antibody
    • BSF 2 antibody
    • BSF-2 antibody
    • BSF2 antibody
    • CDF antibody
    • CTL differentiation factor antibody
    • Cytotoxic T cell differentiation factor antibody
    • Hepatocyte stimulating factor antibody
    • Hepatocyte stimulatory factor antibody
    • HGF antibody
    • HSF antibody
    • Hybridoma growth factor antibody
    • Hybridoma growth factor Interferon beta-2 antibody
    • Hybridoma plasmacytoma growth factor antibody
    • IFN-beta-2 antibody
    • IFNB2 antibody
    • IL 6 antibody
    • IL-6 antibody
    • IL6 antibody
    • IL6_HUMAN antibody
    • Interferon beta 2 antibody
    • Interferon beta-2 antibody
    • Interleukin 6 antibody
    • Interleukin 6 (interferon beta 2) antibody
    • Interleukin BSF 2 antibody
    • Interleukin-6 antibody
    see all

Images

References

ab106024 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your inquiry.

The pair of antibodies ab6672 and ab106024 may work together in sELISA, but since we haven't tested them together unfortunately we just aren't sure. Since they are both polyclonal, they will recognize multiple epitopes, but we don't know if any of those epitopes overlap.

We do have 2 pairs of IL6 antibodies in the catalogue that have been tested in sELISA, but the corresponding detection antibodies are biotinylated. You would have to use HRP-Strep in this case. Unfortunately we don't have an HRP-conjugated IL6 that we know works well in sELISA. It sounds like you're detecting human IL6, so the second set is probably best.

ab9730 and ab84270
https://www.abcam.com/il6-antibody-ab9730.html
https://www.abcam.com/ab84270.html

ab9324 and ab84251
https://www.abcam.com/il6-antibody-ab9324.html
https://www.abcam.com/ab84251.html

The standard ab134672 should work well.

This is our recommended sELISA protocols (with buffer recommendations).

https://www.abcam.com/10x-phosphate-buffered-saline-ab128983.html

We also have ELISA kits where the captture antibody is already precoated: https://www.abcam.com/il6-high-sensitivity-human-elisa-kit-ab46042.html

I hope this information helps. Please contact us with any other questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up