Overview

  • Product name

  • Description

    Rabbit polyclonal to IL-6R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human IL-6R aa 253-267.
    Database link: P08887

  • Positive control

    • WB: Jurkat and 803 cell lysates. IHC-P: Human spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab128008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/500. Predicted molecular weight: 52 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1 - 1/40000.

Target

  • Function

    Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
    Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
  • Tissue specificity

    Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Domain

    The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
  • Post-translational
    modifications

    A short soluble form may also be released from the membrane by proteolysis.
  • Cellular localization

    Secreted and Basolateral cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 126 antibody
    • CD126 antibody
    • CD126 antigen antibody
    • gp80 antibody
    • IL 6R 1 antibody
    • IL 6R alpha antibody
    • IL 6R antibody
    • IL-6 receptor alpha chain antibody
    • IL-6 receptor subunit alpha antibody
    • IL-6R 1 antibody
    • IL-6R subunit alpha antibody
    • IL-6R-alpha antibody
    • IL-6RA antibody
    • IL6Q antibody
    • Il6r antibody
    • IL6RA antibody
    • IL6RA_HUMAN antibody
    • IL6RQ antibody
    • Interleukin 6 receptor antibody
    • interleukin 6 receptor, alpha antibody
    • Interleukin-6 receptor subunit alpha antibody
    • Membrane glycoprotein 80 antibody
    • MGC30256 antibody
    see all

Images

  • Paraffin-embedded human spleen tissue stained for IL6R with ab128008 at 2.5 µg/ml in immunohistochemical analysis.

  • All lanes : Anti-IL-6R antibody (ab128008) at 1/200 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 2 : 803 cell lysate

    Predicted band size: 52 kDa

  • ab128008 staining IL6R in Human Muller cell line MIO-M1 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody (1/400).

    See Abreview

References

This product has been referenced in:

  • Zhang N  et al. Decreased expression of microRNA-30b promotes the development of pulpitis by upregulating the expression of interleukin-6 receptor. Exp Ther Med 17:3233-3238 (2019). Read more (PubMed: 30936998) »
  • Wiegertjes R  et al. TGF-ß dampens IL-6 signaling in articular chondrocytes by decreasing IL-6 receptor expression. Osteoarthritis Cartilage N/A:N/A (2019). Read more (PubMed: 31054955) »
See all 11 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Muller cell line MIO-M1)
Permeabilization
Yes - 0.1% TX-100
Specification
Muller cell line MIO-M1
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 02 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (HeLa cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
No
Specification
HeLa cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
10% NBF

Abcam user community

Verified customer

Submitted Aug 13 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus Monkey Tissue sections (macaque retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6.0
Permeabilization
No
Specification
macaque retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 13 2015

Answer

Thank you for taking time to share that information with me.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been more successful. If you would not mind sharing some images of the staining that would be very helpful to aid in understanding the problems encountered further.

Having reviewed the protocol details, I believe this product should have given satisfactory results. It is clear you have tried to optimise the conditions extensively. It unfortunately appears that you may have received a faulty vial. I apologize for the inconvenience this has caused you. I would be pleased to offer you a replacement vial of this antibody (from a different lot number), or if you would prefer, a different antibody altogether, such as ab47215. Unfortunately, ab47215 has only been used in IHC using frozen sections as yet. This does not mean that it would not work with paraffin embedded sections but as we have not tried it I cannot guarantee its performance. Alternatively, I can arrange for a credit note, or refund in compensation.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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Answer

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer XXXXXX und sollte am 22. Mai bei Ihnen ankommen. Natürlich wird der Ersatzantikörper auch voll von unserem Abpromise abgedeckt!

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