Recombinant
RabMAb

Recombinant Anti-IL-6R antibody [EPR22628-236] (ab222101)

Overview

  • Product name

    Anti-IL-6R antibody [EPR22628-236]
    See all IL-6R primary antibodies
  • Description

    Rabbit monoclonal [EPR22628-236] to IL-6R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human IL-6R aa 1-350. The exact sequence is proprietary.
    Database link: P08887

  • Positive control

    • WB: U266B1 whole cell lysate. IP: U266B1 whole cell lysate. Flow cyt: U937 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab222101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 80, 70, 50, 40 kDa (predicted molecular weight: 52 kDa).
Flow Cyt 1/50.
IP 1/30.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
      Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
    • Tissue specificity

      Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
    • Sequence similarities

      Belongs to the type I cytokine receptor family. Type 3 subfamily.
      Contains 1 fibronectin type-III domain.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    • Domain

      The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    • Post-translational
      modifications

      A short soluble form may also be released from the membrane by proteolysis.
    • Cellular localization

      Secreted and Basolateral cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD 126 antibody
      • CD126 antibody
      • CD126 antigen antibody
      • gp80 antibody
      • IL 6R 1 antibody
      • IL 6R alpha antibody
      • IL 6R antibody
      • IL-6 receptor alpha chain antibody
      • IL-6 receptor subunit alpha antibody
      • IL-6R 1 antibody
      • IL-6R subunit alpha antibody
      • IL-6R-alpha antibody
      • IL-6RA antibody
      • IL6Q antibody
      • Il6r antibody
      • IL6RA antibody
      • IL6RA_HUMAN antibody
      • IL6RQ antibody
      • Interleukin 6 receptor antibody
      • interleukin 6 receptor, alpha antibody
      • Interleukin-6 receptor subunit alpha antibody
      • Membrane glycoprotein 80 antibody
      • MGC30256 antibody
      see all

    Images

    • All lanes : Anti-IL-6R antibody [EPR22628-236] (ab222101) at 1/1000 dilution

      Lane 1 : U266B1 (human multiple myeloma b lymphocyte) whole cell lysate
      Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 52 kDa
      Observed band size: 40,50,70,80 kDa
      why is the actual band size different from the predicted?


      Exposure time: 48 seconds


      The molecular weight observed is consistent with what has been described in the literature (PMID: 28276471, 28060820).

      Bands between 40-70KD are likely to be cleaved forms (PMID: 28060820).

      Negative control: K-562 (PMID: 8300137)

    • IL-6R was immunoprecipitated from 0.35 mg of U266B1 (human multiple myeloma B lymphocyte cell line) whole cell lysate with ab222101 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222101 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as the secondary antibody at 1/5000 dilution.

      Lane 1: U266B1 whole cell lysate 10 μg (Input).
      Lane 2: ab222101 IP in U266B1 whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222101 in U266B1 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 15 seconds.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 28276471; PMID: 28060820).

      Bands between 40-70KD are likely to be cleaved forms (PMID: 28060820).

    • Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) (Left) and U937 (human histiocytic lymphoma cell line) (Right) cells labeling IL-6R  with ab222101 at 1/50 (red) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

      Negative control: K562 (PMID: 8300137)

      Gated on viable cells.

       

    References

    ab222101 has not yet been referenced specifically in any publications.

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