Application
Western blot
Sample
Human Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS Tween 0,5% Lait 5% NaN3
Dilution
1/200
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Clarity
Exposure
30 second(s)
Bands
Specific: 15 kDa Non-specific: 35 kDa
Additional data
Additional Notes
Lane 1: ladder
Lane 2: Human intestinal tissue lysate
Lane 3: Human MCF7 Whole Cell Lysate (specific band at 20kDa, non specific bands at 50,80,160kDa approximatively
Abcam response
Increasing the amount of protein loaded can enhance the signal.
The human IL7 produces two isoforms at 15 and 20 kDa, explaining the difference in the band size in this Western blot (http://www.uniprot.org/uniprot/P13232#sequences).
Mr. Julien Chevalier
Verified customer
Submitted Jul 11 2016