FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Constituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.25 - 1 µg/ml.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 20 kDa.|
FunctionHematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.
Sequence similaritiesBelongs to the IL-7/IL-9 family.
- Information by UniProt
- IL 7 antibody
- IL-7 antibody
- Il7 antibody
Western blot analysis of varying levels of Recombinant rat IL-7 protein using ab271243 at 0.2 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant IL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Rat IL-7 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271243. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/wel of recombinant Rat IL-7 protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271243 has not yet been referenced specifically in any publications.