Anti-IL-8 antibody [807] (ab18672)

Thank you for contacting us.
1. If you prefer to do the incubation at room temperature you should not exceed a 2 hour incubation. We do recommend an overnight incubation at 4 degrees, however, as this usually gives the strongest and most specific staining.
2. I would suggest you use ab6672 at 1/400 and ab18672 at 1/2000.
3. I believe lung cancer tissue is a good positive control for these antibodies but am not sure that the levels in normal tissue are high enough. For ab18672 we have seen good expression in lymph node, poocine epithelial tissue, and human temporal artery. For ab6672 human and mouse pancreatic cancer, head and neck tumor, and human liver sections have shown good results. Any tissue reported in the literature to have good IL6 or IL8 expression should be a good positive control if you do not have access to these tissues.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your reply.

I fully understand your concerns regarding the product code typing error on the datasheet, it is regrettable. I would like to reassure you that the image is from ab18672 IL8 antibody [807], and came from an Abreview submitted by a customer which is published on the Abreview page of the datasheet:

https://www.abcam.com/IL-8-antibody-807-ab18672.html&tab=abreviews&intabreviewid=24612

I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in many cases, regrettably we do not have access to all the articles mentioning our products, or subscriptions to all the journals that publish them. I'm sorry there are no others to provide on this occasion for ab18672.

Regarding the product quality, I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Regrettably, I can suggest you have received a bad vial on this occasion. Therefore, we would be pleased to provide a replacement from the same antibody, which will still be covered by our guarantee. Alternatively, I fully understand your concerns and if you prefer an alternative replacement or credit note in this case, I will be pleased to arrange this for you.

I hope this will be helpful. I look forward to hearing from you with details of how you would like to proceed.

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments, and would like to offer a free of charge replacement or credit note in compensation, providing the product has been used in a tested and guaranteed species. Before arranging this, I would appreciate if you could confirm which species the samples are from?

Thank you for your continued cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you for your enquiry.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

With regards to the text with the image, Ihave investigated this with my colleagues from our datasheetsteam and I can confirm that regrettably there was a typing error. I can confirm this IHC image is fromab18672 Anti-IL8 antibody [807].The datasheet has been updated with the correct information and I apologize for any confusion.

The quality of our antibodies and datasheets is very important to us, as is any feedback and data we receive. It is regrettable that the information we had with the image on this datasheet was not of the standard both we and our customers expect from our products.

With regards to the difficulty with staining, I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forIHC-P and in Human andPig samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you are also able to provide an image which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.



Order Details
Antibody code:

Lot number:


Purchase order number
or preferably Abcam order number:


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, low signal, non-specific satining etc.)

Sample (Species/Tissue/Cell Type/Cell Line etc.)


Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


Permeabilization step


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)


Optimization attempts (problem solving)
How many times have you tried the IHC?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No


What steps have you altered?


Additional Notes


We would appreciate if you are also able to provide and image which woudl help us to assess the results

Thank you for contacting us.

Unfortunately there is no information available about the epitope recognised by these two antibodies, as it hasn’t been mapped.

I am very sorry I couldn’t help you further. Please do not hesitate to contact us if you need any other advice or information.