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    il-8-antibody-807-ab18672.pdf

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Cardiovascular Angiogenesis Cytokines Interleukin
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Anti-IL-8 antibody [807] (ab18672)

  • Datasheet
  • SDS
Reviews (9)Q&A (15)References (45)

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Western blot - Anti-IL-8 antibody [807] (ab18672)

    Key features and details

    • Mouse monoclonal [807] to IL-8
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

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    Biotin Anti-IL-6 antibody (ab84251)

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    Overview

    • Product name

      Anti-IL-8 antibody [807]
      See all IL-8 primary antibodies
    • Description

      Mouse monoclonal [807] to IL-8
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant full length protein corresponding to Human IL-8. Purified recombinant human IL-8.

    • Positive control

      • Purchase matching WB positive control:Recombinant human IL-8 protein
    • General notes

      This product was changed from ascites to tissue culture supernatant on 12/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Constituent: 0.0268% PBS
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Purification notes

      Purified from TCS.
    • Clonality

      Monoclonal
    • Clone number

      807
    • Myeloma

      Sp2/0-Ag14
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Cytokines
      • Interleukin
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • SARS Coronavirus
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Angiogenic growth factors
      • Neuroscience
      • Processes

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant human IL-8 protein (Active) (ab259397)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab18672 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 0.02 - 0.1 µg/ml. Predicted molecular weight: 12 kDa.

    Concentration of 0.02-0.1 ug/ml of ab18672 will allow visualization of 100 ng/lane of human IL-8

    Notes
    WB
    Use a concentration of 0.02 - 0.1 µg/ml. Predicted molecular weight: 12 kDa.

    Concentration of 0.02-0.1 ug/ml of ab18672 will allow visualization of 100 ng/lane of human IL-8

    Target

    • Function

      IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
    • Sequence similarities

      Belongs to the intercrine alpha (chemokine CxC) family.
    • Post-translational
      modifications

      Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P10145 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3576 Human
      • Omim: 146930 Human
      • SwissProt: P10145 Human
      • Unigene: 624 Human
      • Alternative names

        • (Ala-IL-8)77 antibody
        • (Ser-IL-8)72 antibody
        • 9E3 antibody
        • Beta thromboglobulin like protein antibody
        • C-X-C motif chemokine 8 antibody
        • CEF-4 antibody
        • chemokine, CXC motif, ligand 8 antibody
        • CXCL8 antibody
        • Emoctakin antibody
        • GCP-1 antibody
        • GCP/IL-8 protein I antibody
        • GCP/IL-8 protein II antibody
        • GCP/IL-8 protein III antibody
        • GCP/IL-8 protein IV antibody
        • GCP/IL-8 protein V antibody
        • GCP/IL-8 protein VI antibody
        • GCP1 antibody
        • Granulocyte chemotactic protein 1 antibody
        • IL-8 antibody
        • IL-8(1-77) antibody
        • IL-8(9-77) antibody
        • IL8 antibody
        • IL8/NAP1 form I antibody
        • IL8/NAP1 form II antibody
        • IL8/NAP1 form III antibody
        • IL8/NAP1 form IV antibody
        • IL8/NAP1 form V antibody
        • IL8/NAP1 form VI antibody
        • IL8_HUMAN antibody
        • Inteleukin 8 antibody
        • LECT antibody
        • LUCT antibody
        • Lymphocyte-derived neutrophil-activating factor antibody
        • LYNAP antibody
        • MDNCF antibody
        • MDNCF-b antibody
        • MDNCF-c antibody
        • MONAP antibody
        • Monocyte derived neutrophil activating peptide antibody
        • Monocyte derived neutrophil chemotactic factor antibody
        • Monocyte-derived neutrophil chemotactic factor antibody
        • Monocyte-derived neutrophil-activating peptide antibody
        • NAF antibody
        • NAP 1 antibody
        • NAP-1 antibody
        • NAP1 antibody
        • Neutrophil activating peptide 1 antibody
        • Neutrophil activating protein 1 antibody
        • Neutrophil-activating factor antibody
        • Neutrophil-activating protein 1 antibody
        • Protein 3 10C antibody
        • Protein 3-10C antibody
        • SCYB 8 antibody
        • SCYB8 antibody
        • Small inducible cytokine subfamily B member 8 antibody
        • T cell chemotactic factor antibody
        • T-cell chemotactic factor antibody
        • TSG 1 antibody
        • TSG1 antibody
        see all

      Images

      • Western blot - Anti-IL-8 antibody [807] (ab18672)
        Western blot - Anti-IL-8 antibody [807] (ab18672)
        Anti-IL-8 antibody [807] (ab18672) at 0.1 µg/ml + recombinant human IL8, 100 ng

        Predicted band size: 12 kDa



        This image was produced useing a version of the antibody producted in ascites.

      Protocols

      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (45)

      Publishing research using ab18672? Please let us know so that we can cite the reference in this datasheet.

      ab18672 has been referenced in 45 publications.

      • Zhu J  et al. MicroRNA-140-5p regulates the proliferation, apoptosis and inflammation of RA FLSs by repressing STAT3. Exp Ther Med 21:171 (2021). PubMed: 33456538
      • Su H  et al. Identification of hub genes associated with neutrophils infiltration in colorectal cancer. J Cell Mol Med 25:3371-3380 (2021). PubMed: 33666342
      • Zhang S  et al. Activation of Toll-Like Receptor 7 Signaling Pathway in Primary Sjögren's Syndrome-Associated Thrombocytopenia. Front Immunol 12:637659 (2021). PubMed: 33767707
      • Zhou Z  et al. LncRNA XIST promotes inflammation by downregulating GRa expression in the adenoids of children with OSAHS. Exp Ther Med 21:500 (2021). PubMed: 33791009
      • Jin H  et al. Natural killer cells inhibit breast cancer cell invasion through downregulation of urokinase-type plasminogen activator. Oncol Rep 45:299-308 (2021). PubMed: 33155661
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 24 Abreviews or Q&A

      Western blot abreview for Anti-IL-8 antibody [807]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Dopaminergic Neurons & HEK-293T)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      30 µg
      Specification
      Dopaminergic Neurons & HEK-293T
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Nov 16 2020

      Western blot abreview for Anti-IL-8 antibody [807]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (kidney)
      Gel Running Conditions
      Reduced Denaturing (4-12% bis-tris gel, MES buffer)
      Loading amount
      30 µg
      Specification
      kidney
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Nov 03 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-8 antibody [807]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (PC3 Prostate cancer cells)
      Permeabilization
      Yes - 0.1% Triton X-100
      Specification
      PC3 Prostate cancer cells
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Jan 30 2019

      ELISA abreview for Anti-IL8 antibody [807]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      ELISA
      Sample
      Human Recombinant protein (Human IL-8 from R&D Systems)
      Specification
      Human IL-8 from R&D Systems
      Type
      Sandwich (Capture)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Mar 07 2014

      Immunohistochemistry (Frozen sections) abreview for Anti-IL8 antibody [807]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
      Sample
      Human Tissue sections (tumor)
      Specification
      tumor
      Permeabilization
      Yes - PBS+0.3% Triton-X-100
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Dec 06 2013

      Question

      I want to compare the
      expression of IL-6 & IL-8 in human nasal polyp tissue (parffin embeded tissue).I recently purchased some Abcam IL-8 antibody (ab18672) and some Abcam IL-6 antibody (ab6672).I have no experience in IHC-P and I am faced with limited financial budgets,so I can not risk.
      I have used it as per the recommendations in the product data sheet but am still having problems and questions I hope you can address.
      1- I want to do my test in room temperature. what is your recommendation about this two primary antibodies incubation time?
      2- what is the Appropriate dilution for ab18672 and ab6672?
      3- If possible could you also suggest a good positive control tissue for IHC and this antibody? Is the lung tissue suitable?
      Any help and answers will be greatly appreciated.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 07 2013

      Answer

      Thank you for contacting us.
      1. If you prefer to do the incubation at room temperature you should not exceed a 2 hour incubation. We do recommend an overnight incubation at 4 degrees, however, as this usually gives the strongest and most specific staining.
      2. I would suggest you use ab6672 at 1/400 and ab18672 at 1/2000.
      3. I believe lung cancer tissue is a good positive control for these antibodies but am not sure that the levels in normal tissue are high enough. For ab18672 we have seen good expression in lymph node, poocine epithelial tissue, and human temporal artery. For ab6672 human and mouse pancreatic cancer, head and neck tumor, and human liver sections have shown good results. Any tissue reported in the literature to have good IL6 or IL8 expression should be a good positive control if you do not have access to these tissues.
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Jan 07 2013

      Question

      Dear Kate

      Thank you for your prompt response, I will pass the message to my supervisors. All of the samples we have tested the antibody on were human.

      So far we have not been able to find a publication where Abcam antibody ab18672 has been used for immunohistochemistry on paraffin embedded tissue. After finding the error in the Abcam product information sheet I would prefer to use an antibody that has been shown to work. Could you provide any evidence or publications were ab18672 has been used successfully for IHC on paraffin embedded tissue?

      Ideally we would like a refund so we can purchase an antibody we have seen in used on published data.

      Best Regards

      Read More

      Abcam community

      Verified customer

      Asked on Nov 14 2012

      Answer

      Thank you for your reply.

      I fully understand your concerns regarding the product code typing error on the datasheet, it is regrettable. I would like to reassure you that the image is from ab18672 IL8 antibody [807], and came from an Abreview submitted by a customer which is published on the Abreview page of the datasheet:

      https://www.abcam.com/IL-8-antibody-807-ab18672.html&tab=abreviews&intabreviewid=24612

      I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in many cases, regrettably we do not have access to all the articles mentioning our products, or subscriptions to all the journals that publish them. I'm sorry there are no others to provide on this occasion for ab18672.

      Regarding the product quality, I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Regrettably, I can suggest you have received a bad vial on this occasion. Therefore, we would be pleased to provide a replacement from the same antibody, which will still be covered by our guarantee. Alternatively, I fully understand your concerns and if you prefer an alternative replacement or credit note in this case, I will be pleased to arrange this for you.

      I hope this will be helpful. I look forward to hearing from you with details of how you would like to proceed.

      Read More

      Abcam Scientific Support

      Answered on Nov 14 2012

      Question

      Dear AbCam Technical Support

      We have conducted further tests and are confident in our selves there is a problem with the antibody we have.

      Please see below for the requested information (black) and our response (red).

      Kind Regards

      Read More

      Abcam community

      Verified customer

      Asked on Nov 12 2012

      Answer

      Thank you for your message and for providing this further information.

      I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments, and would like to offer a free of charge replacement or credit note in compensation, providing the product has been used in a tested and guaranteed species. Before arranging this, I would appreciate if you could confirm which species the samples are from?

      Thank you for your continued cooperation. I look forward to hearing from you with details of how you would like to proceed.

      Read More

      Abcam Scientific Support

      Answered on Nov 12 2012

      Question

      Dear Abcam Technical Support

      I recently purchased some Abcam IL-8 antibody (ab18672). I have used it as per the recommendations in the product data sheet but am still having problems and questions I hope you can address.

      I have stained paraffin embedded ovarian tumour microarrays using immunohistochemistry (IHC) with a Novolink detection kit. I purchased three other Abcam antibodies at the same time of which the staining has worked perfectly and are being analysed now. I have not had any IL-8 staining in the ovarian tissue; a literature search suggested kidney epithelium is an appropriate positive control however, when repeated on kidney tissue section I have had no luck.

      On closer inspection of the data sheet available online and delivered with the antibody (attached), there is an example of inflamed human temporal artery using IHC on paraffin embedded tissue. The figure is labelled “ab18675 staining IL8 in inflammed human temporal artery by Immunohistochemistry” . ab18675 is an Abcam code for IL-6. Is temporal artery a positive control for IL-6 or IL-8?

      Can you provide any references or evidence that the antibody I have purchased (Ab18672) is compatible with IHC on paraffin sections?

      Neither of the references provided use IHC with this antibody, though IHC-P is listed as a tested application.

      If possible could you also suggest a good positive control tissue for IHC and this antibody?

      Any help and answers will be greatly appreciated.

      Regards

      Read More

      Abcam community

      Verified customer

      Asked on Sep 28 2012

      Answer

      Thank you for your enquiry.

      Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

      With regards to the text with the image, Ihave investigated this with my colleagues from our datasheetsteam and I can confirm that regrettably there was a typing error. I can confirm this IHC image is fromab18672 Anti-IL8 antibody [807].The datasheet has been updated with the correct information and I apologize for any confusion.

      The quality of our antibodies and datasheets is very important to us, as is any feedback and data we receive. It is regrettable that the information we had with the image on this datasheet was not of the standard both we and our customers expect from our products.

      With regards to the difficulty with staining, I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forIHC-P and in Human andPig samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

      I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

      I would appreciate if you are also able to provide an image which would help us to assess the results.

      Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.



      Order Details
      Antibody code:

      Lot number:


      Purchase order number
      or preferably Abcam order number:


      General Information
      Antibody storage conditions (temperature/reconstitution etc)


      Description of the problem (high background, low signal, non-specific satining etc.)

      Sample (Species/Tissue/Cell Type/Cell Line etc.)


      Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


      Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


      Permeabilization step


      Blocking conditions (Buffer/time period, Blocking agent etc.)


      Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


      Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


      Detection method


      Positive and negative controls used (please specify)


      Optimization attempts (problem solving)
      How many times have you tried the IHC?



      Have you run a "No Primary" control?
      Yes No

      Do you obtain the same results every time?
      Yes No


      What steps have you altered?


      Additional Notes


      We would appreciate if you are also able to provide and image which woudl help us to assess the results

      Read More

      Abcam Scientific Support

      Answered on Sep 28 2012

      Question

      Dear all,
      We had a look and ab1793 and ab18672 seem to look the most suitable. Would you be able to please e-mail him the epitopes of these monoclonals

      Read More

      Abcam community

      Verified customer

      Asked on Sep 11 2012

      Answer

      Thank you for contacting us.

      Unfortunately there is no information available about the epitope recognised by these two antibodies, as it hasn’t been mapped.

      I am very sorry I couldn’t help you further. Please do not hesitate to contact us if you need any other advice or information.

      Read More

      Abcam Scientific Support

      Answered on Sep 11 2012

      1-10 of 24 Abreviews or Q&A

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