Anti-IL-8 antibody [807] (ab18672)
- Datasheet
- References (16)
- Protocols
Overview
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Product nameAnti-IL-8 antibody [807]
See all IL-8 primary antibodies -
DescriptionMouse monoclonal [807] to IL-8
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Host speciesMouse
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Tested applicationsSuitable for: ELISA, Neutralising, WB, IHC-Fr, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human, Pig
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Immunogen
Purified recombinant human IL-8.
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Positive control
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General notes
This product was changed from ascites to tissue culture supernatant on 12/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Constituent: 0.0268% PBS -
Concentration information loading... -
PurityAscites
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Purification notesPurified from ascites.
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ClonalityMonoclonal
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Clone number807
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MyelomaSp2/0-Ag14
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab18672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| ELISA | Use at an assay dependent concentration. | |
| Neutralising | Use at an assay dependent concentration. In chemotaxis assay, the antibody inhibited 95.1% of the chemotactic activity of IL-8 on RB/293 cell. 50ug/ml of antibody and 10ng/ml of human IL-8 were used in assay. This antibody inhibited the chemotactic effect of human IL-8, and had no inhibitory effect of MIP 1-beta and RANTES in neutrophil chemotaxis assay, indicating that the neutralizing activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes. |
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| WB | Use a concentration of 0.02 - 0.1 µg/ml. Predicted molecular weight: 12 kDa. Concentration of 0.02-0.1 ug/ml of ab18672 will allow visualization of 100 ng/lane of human IL-8 |
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| IHC-Fr | 1/2000. | |
| IHC-P | 1/2000. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 18697197 |
Target
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FunctionIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
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Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
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Post-translational
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 3576 Human
- Entrez Gene: 396880 Pig
- Omim: 146930 Human
- SwissProt: P10145 Human
- SwissProt: P26894 Pig
- Unigene: 624 Human
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Alternative names
- (Ala-IL-8)77 antibody
- (Ser-IL-8)72 antibody
- 9E3 antibody
see all
Images
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![Western blot - Anti-IL-8 antibody [807] (ab18672)](https://www.abcam.com/ps/products/18/ab18672/Images/ab18672-299389-anti-il-8-antibody-807-western-blot.jpg)
Western blot - Anti-IL-8 antibody [807] (ab18672)Anti-IL-8 antibody [807] (ab18672) at 0.1 µg/ml + recombinant human IL8, 100 ng
Predicted band size: 12 kDaThis image was produced useing a version of the antibody producted in ascites.
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![Immunohistochemistry (Frozen sections) - Anti-IL-8 antibody [807] (ab18672)](https://www.abcam.com/ps/products/18/ab18672/Images/ab18672-28231-anti-il-8-antibody-807-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-IL-8 antibody [807] (ab18672)This image is courtesy of Daniel Holod-Betz by Abreview.ab18672 staining IL8 in porcine epithelial tissue by Immunohistochemistry (Frozen sections). Tissue was fixed in paraformaldehyde, permeabilized using 0.25% Triton X-100, blocked with 10% serum for 2 hours at 25°C, then incubated with ab18672 at a 1/2000 dilution for 12 hours at 4°C. The secondary used was ab98640, used at a 1/1000 dilution.
This image was produced useing a version of the antibody producted in ascites.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody [807] (ab18672)](https://www.abcam.com/ps/products/18/ab18672/Images/ab18672-28232-anti-il-8-antibody-807-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody [807] (ab18672)Image courtesy of an anonymous Abreview.ab18672 staining IL8 in inflammed human temporal artery by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in formaldehyde and an antigen retrieval step was performed using sodium citrate. Samples were then blocked with 5% serum for 1 hour at 25°C, then incubated with ab18672 at a 1/2000 dilution for 16 hours at 4°C. The secondary used was a rabbit anti-mouse polyclonal conjugated to biotin, 1/1000 dilution.
This image was produced useing a version of the antibody producted in ascites.
Protocols
References
This product has been referenced in:
- Nuzzo D et al. Glucagon-like peptide-2 reduces the obesity-associated inflammation in the brain. Neurobiol Dis 121:296-304 (2019). Read more (PubMed: 30347266) »
- Higashino N et al. Fibroblast activation protein-positive fibroblasts promote tumor progression through secretion of CCL2 and interleukin-6 in esophageal squamous cell carcinoma. Lab Invest N/A:N/A (2019). Read more (PubMed: 30683902) »
