Overview

  • Product name
    Anti-IL-8 antibody [807]
    See all IL-8 primary antibodies
  • Description
    Mouse monoclonal [807] to IL-8
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, Neutralising, WB, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Purified recombinant human IL-8.

  • Positive control
  • General notes

    This product was changed from ascites to tissue culture supernatant on 12/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab18672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.

In chemotaxis assay, the antibody inhibited 95.1% of the chemotactic activity of IL-8 on RB/293 cell. 50ug/ml of antibody and 10ng/ml of human IL-8 were used in assay. This antibody inhibited the chemotactic effect of human IL-8, and had no inhibitory effect of MIP 1-beta and RANTES in neutrophil chemotaxis assay, indicating that the neutralizing activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes.

WB Use a concentration of 0.02 - 0.1 µg/ml. Predicted molecular weight: 12 kDa.

Concentration of 0.02-0.1 ug/ml of ab18672 will allow visualization of 100 ng/lane of human IL-8

IHC-Fr 1/2000.
IHC-P 1/2000.
ICC/IF Use at an assay dependent concentration. PubMed: 18697197

Target

  • Function
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 9E3 antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • LECT antibody
    • LUCT antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG1 antibody
    see all

Images

  • Anti-IL-8 antibody [807] (ab18672) at 0.1 µg/ml + recombinant human IL8, 100 ng

    Predicted band size: 12 kDa



    This image was produced useing a version of the antibody producted in ascites.

  • ab18672 staining IL8 in porcine epithelial tissue by Immunohistochemistry (Frozen sections). Tissue was fixed in paraformaldehyde, permeabilized using 0.25% Triton X-100, blocked with 10% serum for 2 hours at 25°C, then incubated with ab18672 at a 1/2000 dilution for 12 hours at 4°C. The secondary used was ab98640, used at a 1/1000 dilution.

    This image was produced useing a version of the antibody producted in ascites.

    See Abreview

  • ab18672 staining IL8 in inflammed human temporal artery by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in formaldehyde and an antigen retrieval step was performed using sodium citrate. Samples were then blocked with 5% serum for 1 hour at 25°C, then incubated with ab18672 at a 1/2000 dilution for 16 hours at 4°C. The secondary used was a rabbit anti-mouse polyclonal conjugated to biotin, 1/1000 dilution.

    This image was produced useing a version of the antibody producted in ascites.

    See Abreview

References

This product has been referenced in:
  • Nuzzo D  et al. Glucagon-like peptide-2 reduces the obesity-associated inflammation in the brain. Neurobiol Dis 121:296-304 (2019). Read more (PubMed: 30347266) »
  • Higashino N  et al. Fibroblast activation protein-positive fibroblasts promote tumor progression through secretion of CCL2 and interleukin-6 in esophageal squamous cell carcinoma. Lab Invest N/A:N/A (2019). Read more (PubMed: 30683902) »
See all 16 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-7 of 7 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PC3 Prostate cancer cells)
Permeabilization
Yes - 0.1% Triton X-100
Specification
PC3 Prostate cancer cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 30 2019

Application
ELISA
Sample
Human Recombinant protein (Human IL-8 from R&D Systems)
Specification
Human IL-8 from R&D Systems
Type
Sandwich (Capture)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 07 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample
Human Tissue sections (tumor)
Specification
tumor
Permeabilization
Yes - PBS+0.3% Triton-X-100
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 06 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Rhesus Lymph Node)
Specification
Rhesus Lymph Node
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Apr 02 2012

Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Epithelial)
Specification
Epithelial
Fixative
Paraformaldehyde
Permeabilization
Yes - .025% Triton X100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Mr. Daniel Holod-Betz

Verified customer

Submitted Nov 11 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Brain Adventitial Fibroblasts)
Specification
Human Brain Adventitial Fibroblasts
Fixative
Formaldehyde
Permeabilization
Yes - Triton-X 0.3%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 30 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Inflamed Human Temporal Artery)
Specification
Inflamed Human Temporal Artery
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 06 2011

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