Overview

  • Product name
    Anti-IL-8 antibody [8M6]
    See all IL-8 primary antibodies
  • Description
    Mouse monoclonal [8M6] to IL-8
  • Host species
    Mouse
  • Specificity
    Recognises ovine interleukin-8 and shows no cross-reactivity with ovine IL1 beta, IL6, MCP 1 or TNF alpha.
  • Tested applications
    Suitable for: Flow Cyt, WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Sheep, Rabbit, Cow, Dog, Pig
  • Immunogen

    Recombinant full length protein corresponding to Sheep IL-8.

  • General notes


    Neutralises the bioactivity of ovine IL8 (removal of sodium azide is recommended prior to use in functional assays).

Properties

Applications

Our Abpromise guarantee covers the use of ab34100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.

Use 10µl of the suggested working dilution to label 106 cells. Membrane permeabilisation is required.

 

 

 

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
ELISA Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 21494875

Target

  • Function
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 9E3 antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • LECT antibody
    • LUCT antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG1 antibody
    see all

References

This product has been referenced in:
  • Wu X  et al. Inflammatory immune response in rabbits with Staphylococcus aureus biofilm-associated sinusitis. Int Forum Allergy Rhinol N/A:N/A (2018). Read more (PubMed: 29979838) »
  • Tang Z  et al. Gene Expression Profiling of Pulmonary Artery in a Rabbit Model of Pulmonary Thromboembolism. PLoS One 11:e0164530 (2016). Read more (PubMed: 27798647) »
See all 8 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Goat Cultured Cells (Nucleus Populous)
Permeabilization
Yes - 3% Triton-100
Specification
Nucleus Populous
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Chenghao Zhang

Verified customer

Submitted Dec 12 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Goat Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
Permeabilization
Yes - 3% Hydrogen Peroxide
Specification
Lung
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Chenghao Zhang

Verified customer

Submitted Dec 10 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Heart, Liver, lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: PH6 and PH9
Permeabilization
No
Specification
Heart, Liver, lymph node
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 23 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA buffer pH 8.0. in pressure cooker
Sample
Cow Tissue sections (Ovary)
Specification
Ovary
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Dr. Natalia Salvetti

Verified customer

Submitted Oct 09 2014

Answer

DISCOUNT CODES:
***

Expiration date: November 26, 2012

These codes will each give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for each antibody and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Eachcode will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your call today and for your interest in our IL6 and IL8 antibodies.

I've looked over the rest of our IL6 antibodies, and we unfortunately don't have any that Istrongly expect to cross-react with rabbit samples. The mouse monoclonal ab9324 could cross-react, as it does react with rat samples which are also dissimilar to the immunogen human sequence.

We also have a rabbit polyclonal antibody, ab6672, which was raised against the full-length human protein and may have a higher chance of cross-reaction since it is polyclonal. However if you willbe staining tissue with expected endogenous IgG molecules (i.e. spleen or tonsil) thentherecan behigh backgroundfrom reaction between the tissue and the anti-rabbit secondary antibody. I'm not sure which IL6 antibodywill be the better option for you to try, but if you would like to try either of these, we do have a testing discount program (see details below for ab34100) that you may be interested in.

Since the IL8 antibody ab34100 has not been tested in IHC-P,I can offer a discount off a future purchase if you buy ab34100 now, test it in IHC-P,and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would beworth 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab34100 in IHC-P. I will then send a discount code. This code must be issued before purchasing ab34100 so please wait for my reply before ordering.

2. Purchase ab34100 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-P.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab34100 turns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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