Overview

  • Product name
    Anti-IL-8 antibody [8M6]
    See all IL-8 primary antibodies
  • Description
    Mouse monoclonal [8M6] to IL-8
  • Host species
    Mouse
  • Specificity
    Recognises ovine interleukin-8 and shows no cross-reactivity with ovine IL1 beta, IL6, MCP 1 or TNF alpha.
  • Tested applications
    Suitable for: Flow Cyt, WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Sheep, Rabbit, Cow, Dog, Pig
  • Immunogen

    Recombinant full length protein corresponding to Sheep IL-8.

  • General notes


    Neutralises the bioactivity of ovine IL8 (removal of sodium azide is recommended prior to use in functional assays).

Properties

Applications

Our Abpromise guarantee covers the use of ab34100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.

Use 10µl of the suggested working dilution to label 106 cells. Membrane permeabilisation is required.

 

 

 

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
ELISA Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 21494875

Target

  • Function
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 9E3 antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • LECT antibody
    • LUCT antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG1 antibody
    see all

References

This product has been referenced in:
  • Wu X  et al. Inflammatory immune response in rabbits with Staphylococcus aureus biofilm-associated sinusitis. Int Forum Allergy Rhinol N/A:N/A (2018). Read more (PubMed: 29979838) »
  • Tang Z  et al. Gene Expression Profiling of Pulmonary Artery in a Rabbit Model of Pulmonary Thromboembolism. PLoS One 11:e0164530 (2016). Read more (PubMed: 27798647) »
See all 8 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Application
Immunocytochemistry
Sample
Goat Cultured Cells (Nucleus Populous)
Permeabilization
Yes - 3% Triton-100
Specification
Nucleus Populous
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Chenghao Zhang

Verified customer

Submitted Dec 12 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Goat Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
Permeabilization
Yes - 3% Hydrogen Peroxide
Specification
Lung
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Chenghao Zhang

Verified customer

Submitted Dec 10 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Heart, Liver, lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: PH6 and PH9
Permeabilization
No
Specification
Heart, Liver, lymph node
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 23 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA buffer pH 8.0. in pressure cooker
Sample
Cow Tissue sections (Ovary)
Specification
Ovary
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Dr. Natalia Salvetti

Verified customer

Submitted Oct 09 2014

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