Overview

  • Product name
  • Description
    Rabbit polyclonal to IL-8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human IL-8 aa 77-95 (C terminal).
    Sequence:

    CLDPKENWVQRVVEKFLKR

  • Positive control
    • Human appedicitis tissue. Recombinant Human IL8 protein

Properties

Applications

Our Abpromise guarantee covers the use of ab106350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 11 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 9E3 antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • LECT antibody
    • LUCT antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG1 antibody
    see all

Images

  • All lanes : Anti-IL-8 antibody (ab106350) at 1 µg/ml

    Lane 1 : Recombinant Human IL8 protein at 20ng
    Lane 2 : Recombinant Human IL8 protein at 10ng
    Lane 3 : Recombinant Human IL8 protein at 5ng
    Lane 4 : Raji whole cell lysate
    Lane 5 : MK (55kD)

    Predicted band size: 11 kDa

  • ab106350, at 1µg/ml, staining IL8 in paraffin-embedded Human appedicitis tissue by Immunohistochemistry.

References

This product has been referenced in:
  • Arnandis T  et al. Oxidative Stress in Cells with Extra Centrosomes Drives Non-Cell-Autonomous Invasion. Dev Cell 47:409-424.e9 (2018). Read more (PubMed: 30458137) »
  • Yin L  et al. Ultraviolet B Inhibits IL-17A/TNF-a-Stimulated Activation of Human Dermal Fibroblasts by Decreasing the Expression of IL-17RA and IL-17RC on Fibroblasts. Front Immunol 8:91 (2017). WB . Read more (PubMed: 28217129) »
See all 4 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your email. This inquiry makes more sense now. Currently we do not have anti IL8 products which had been tested in sELISA in combination with other antibodies. I am sorry I am unable to recommend any capture or detection antibody at this moment. Basically, antibodies can only be used in sELISA if they are specific to different epitopes of the immunogen and in order to know the epitopes of few of our monoclonal antibodies I have sent an email to my colleague in lab and now waiting a reply. Once I get an answer I will be able to advice using few of IL8 antibodies in sELISA. Though these antibodies were never tested in sELISA so I would also be happy to offer you a 100% Abreview testing discount which states we will give next purchase of antibody free of charge if you buy the antibodies, test in sELISA and submit a sELISA Abreview. I will get back to you with the information soon.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
For licensing inquiries, please contact partnerships@abcam.com

Sign up