For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
have a nice day
we purchased Anti-IL-8 antibody (ab7747) for immunohistochemistry inparaffin sections IHC-P, it wasn't mentioned what is the diluation that you recommend, you provided 6 references for the product but I have problem with access and publisher asked for money to reveal full text of articles (non of them is free in pubmed).
here I would like to request you to provide us with the recommended titre (dilution of antibody) either you recommend or from the 6 references you provided for the product
thank you for much indeed
Asked on Jun 15 2012
Thank you for your inquiry.
I am happy to confirm that the recommended protocol in IHC is the following:
Antigen retrieval: 5 min proteolytic enzyme (competitor), 20C
Antibody incubation: 120 min, 20C 1:500
I hope this information is helpful and wish you good luck with your experiments.
Answered on Jun 15 2012