Question (50978) | Anti-IL-8 antibody (ab7747)

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Question

Dear Sir,
have a nice day

we purchased Anti-IL-8 antibody (ab7747) for immunohistochemistry inparaffin sections IHC-P, it wasn't mentioned what is the diluation that you recommend, you provided 6 references for the product but I have problem with access and publisher asked for money to reveal full text of articles (non of them is free in pubmed).

here I would like to request you to provide us with the recommended titre (dilution of antibody) either you recommend or from the 6 references you provided for the product

thank you for much indeed

yours,

Answer

Thank you for your inquiry.

I am happy to confirm that the recommended protocol in IHC is the following:

Antigen retrieval: 5 min proteolytic enzyme (competitor), 20C

Antibody incubation: 120 min, 20C 1:500

(http://www.ncbi.nlm.nih.gov/pubmed/17208399?dopt=Abstract)

I hope this information is helpful and wish you good luck with your experiments.

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