Anti-IL-8 antibody [C4] (ab239493)
Key features and details
- Mouse monoclonal [C4] to IL-8
- Suitable for: WB
- Reacts with: Dog, Human
- Isotype: IgG2a
Overview
-
Product name
Anti-IL-8 antibody [C4]
See all IL-8 primary antibodies -
Description
Mouse monoclonal [C4] to IL-8 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Dog, Human -
Immunogen
Recombinant full length protein corresponding to Dog IL-8 aa 1 to the C-terminus. N-terminal His and S tag. Expressed in E. coli. Full length mature chain without signal peptide.
Database link: P10145 -
Positive control
- WB: Recombinant canine IL8 protein; Human leukocyte cells and cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C4 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.5 - 5 µg/ml.
|
Notes |
---|
WB
Use a concentration of 0.5 - 5 µg/ml. |
Target
-
Function
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 403850 Dog
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P41324 Dog
- SwissProt: P10145 Human
- Unigene: 624 Human
-
Alternative names
- (Ala-IL-8)77 antibody
- (Ser-IL-8)72 antibody
- 9E3 antibody
see all
Images
-
Anti-IL-8 antibody [C4] (ab239493) at 5 µg/ml + Recombinant canine IL8 protein
-
Anti-IL-8 antibody [C4] (ab239493) at 4 µg/ml + Human leukocyte cells
Secondary
HRP-Goat Anti-Mouse IgG at 0.2 µg/ml -
Anti-IL-8 antibody [C4] (ab239493) at 4 µg/ml + Human leukocyte cell lysate
Secondary
HRP-Goat Anti-Mouse IgG at 0.2 µg/ml
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab239493 has not yet been referenced specifically in any publications.