Anti-IL-8 antibody [EP1117Y] (ab52612)
![Western blot - Anti-IL-8 antibody [EP1117Y] (ab52612)](https://www.abcam.com/ps/products/52/ab52612/Images/ab52612-64953-anti-il-8-antibody-ep1117y-western-blot.jpg "Western blot - Anti-IL-8 antibody [EP1117Y] (ab52612)")
Key features and details
- Rabbit monoclonal [EP1117Y] to IL-8
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-IL-8 antibody [EP1117Y]
See all IL-8 primary antibodies -
Description
Rabbit monoclonal [EP1117Y] to IL-8 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Human
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Immunogen
Synthetic peptide within Human IL-8 aa 50-150 (C terminal). The exact sequence is proprietary.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1117Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/50000 - 1/100000. Predicted molecular weight: 11 kDa.
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| Notes |
|---|
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WB
1/50000 - 1/100000. Predicted molecular weight: 11 kDa. |
Target
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Function
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
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Alternative names
- (Ala-IL-8)77 antibody
- (Ser-IL-8)72 antibody
- 9E3 antibody
see all
Images
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Western blot - Anti-IL-8 antibody [EP1117Y] (ab52612)Anti-IL-8 antibody [EP1117Y] (ab52612) at 1/200000 dilution + recombinant IL-8
Secondary
Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab52612 has been referenced in 1 publication.
- Laberge RM et al. MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation. Nat Cell Biol 17:1049-61 (2015). WB . PubMed: 26147250
