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Cardiovascular Angiogenesis Cytokines Interleukin
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RabMAb

Anti-IL-8 antibody [EP1117Y] (ab52612)

  • Datasheet
Reviews (1) Submit a question References (1)

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Western blot - Anti-IL-8 antibody [EP1117Y] (ab52612)

    Key features and details

    • Rabbit monoclonal [EP1117Y] to IL-8
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG

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    Overview

    • Product name

      Anti-IL-8 antibody [EP1117Y]
      See all IL-8 primary antibodies
    • Description

      Rabbit monoclonal [EP1117Y] to IL-8
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Recombinant fragment
      See all applications and species data
    • Immunogen

      Synthetic peptide within Human IL-8 aa 50-150 (C terminal). The exact sequence is proprietary.

    • General notes

      Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.20
      Preservative: 0.05% Sodium azide
      Constituents: 99% Tissue culture supernatant, 0.1% BSA
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Clonality

      Monoclonal
    • Clone number

      EP1117Y
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Cytokines
      • Interleukin
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Angiogenic growth factors

    Associated products

    • Isotype control

      • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Recombinant Protein

      • Recombinant human IL-8 protein (Active) (ab259397)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab52612 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Recombinant fragment
    All applications
    Human
    Application Abreviews Notes
    WB
    1/50000 - 1/100000. Predicted molecular weight: 11 kDa.
    Notes
    WB
    1/50000 - 1/100000. Predicted molecular weight: 11 kDa.

    Target

    • Function

      IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
    • Sequence similarities

      Belongs to the intercrine alpha (chemokine CxC) family.
    • Post-translational
      modifications

      Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P10145 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3576 Human
      • Omim: 146930 Human
      • SwissProt: P10145 Human
      • Unigene: 624 Human
      • Alternative names

        • (Ala-IL-8)77 antibody
        • (Ser-IL-8)72 antibody
        • 9E3 antibody
        • Beta thromboglobulin like protein antibody
        • C-X-C motif chemokine 8 antibody
        • CEF-4 antibody
        • chemokine, CXC motif, ligand 8 antibody
        • CXCL8 antibody
        • Emoctakin antibody
        • GCP-1 antibody
        • GCP/IL-8 protein I antibody
        • GCP/IL-8 protein II antibody
        • GCP/IL-8 protein III antibody
        • GCP/IL-8 protein IV antibody
        • GCP/IL-8 protein V antibody
        • GCP/IL-8 protein VI antibody
        • GCP1 antibody
        • Granulocyte chemotactic protein 1 antibody
        • IL-8 antibody
        • IL-8(1-77) antibody
        • IL-8(9-77) antibody
        • IL8 antibody
        • IL8/NAP1 form I antibody
        • IL8/NAP1 form II antibody
        • IL8/NAP1 form III antibody
        • IL8/NAP1 form IV antibody
        • IL8/NAP1 form V antibody
        • IL8/NAP1 form VI antibody
        • IL8_HUMAN antibody
        • Inteleukin 8 antibody
        • LECT antibody
        • LUCT antibody
        • Lymphocyte-derived neutrophil-activating factor antibody
        • LYNAP antibody
        • MDNCF antibody
        • MDNCF-b antibody
        • MDNCF-c antibody
        • MONAP antibody
        • Monocyte derived neutrophil activating peptide antibody
        • Monocyte derived neutrophil chemotactic factor antibody
        • Monocyte-derived neutrophil chemotactic factor antibody
        • Monocyte-derived neutrophil-activating peptide antibody
        • NAF antibody
        • NAP 1 antibody
        • NAP-1 antibody
        • NAP1 antibody
        • Neutrophil activating peptide 1 antibody
        • Neutrophil activating protein 1 antibody
        • Neutrophil-activating factor antibody
        • Neutrophil-activating protein 1 antibody
        • Protein 3 10C antibody
        • Protein 3-10C antibody
        • SCYB 8 antibody
        • SCYB8 antibody
        • Small inducible cytokine subfamily B member 8 antibody
        • T cell chemotactic factor antibody
        • T-cell chemotactic factor antibody
        • TSG 1 antibody
        • TSG1 antibody
        see all

      Images

      • Western blot - Anti-IL-8 antibody [EP1117Y] (ab52612)
        Western blot - Anti-IL-8 antibody [EP1117Y] (ab52612)
        Anti-IL-8 antibody [EP1117Y] (ab52612) at 1/200000 dilution + recombinant IL-8

        Secondary
        Goat anti-rabbit HRP antibody at 1/2000 dilution

        Predicted band size: 11 kDa
        Observed band size: 11 kDa

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab52612? Please let us know so that we can cite the reference in this datasheet.

      ab52612 has been referenced in 1 publication.

      • Laberge RM  et al. MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation. Nat Cell Biol 17:1049-61 (2015). WB . PubMed: 26147250

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL8 antibody [EP1117Y]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric acid
      Sample
      Human Tissue sections (Tonsil)
      Specification
      Tonsil
      Permeabilization
      No
      Fixative
      Formaldehyde
      Read More

      Mr. Carl Hobbs

      Verified customer

      Submitted Nov 03 2014

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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