Anti-IL-8 antibody [EP1117Y] (ab52612)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-IL-8 antibody [EP1117Y]
See all IL-8 primary antibodies -
DescriptionRabbit monoclonal [EP1117Y] to IL-8
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human IL-8 aa 50-150 (C terminal). The exact sequence is proprietary.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP1117Y
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab52612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/50000 - 1/100000. Predicted molecular weight: 11 kDa. |
Target
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FunctionIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
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Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
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Post-translational
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
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Alternative names
- (Ala-IL-8)77 antibody
- (Ser-IL-8)72 antibody
- 9E3 antibody
see all
Images
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![Western blot - Anti-IL-8 antibody [EP1117Y] (ab52612)](https://www.abcam.com/ps/products/52/ab52612/Images/ab52612wb.jpg)
Western blot - Anti-IL-8 antibody [EP1117Y] (ab52612)Anti-IL-8 antibody [EP1117Y] (ab52612) at 1/200000 dilution + recombinant IL-8
Secondary
Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Datasheets and documents
References
This product has been referenced in:
- Laberge RM et al. MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation. Nat Cell Biol 17:1049-61 (2015). WB . Read more (PubMed: 26147250) »
