Product nameAnti-IL-8 antibody [EPR1116(2)]
See all IL-8 primary antibodies
DescriptionRabbit monoclonal [EPR1116(2)] to IL-8
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Human
within Human IL-8 aa 1 to the C-terminus. The exact sequence is proprietary.
- WB: IL8 recombinant protein.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab110727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 11 kDa.|
FunctionIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
- Information by UniProt
- (Ala-IL-8)77 antibody
- (Ser-IL-8)72 antibody
- 9E3 antibody
Anti-IL-8 antibody [EPR1116(2)] (ab110727) at 1/10000 dilution (purified) + Human interleukin 8 recombinant protein at 0.005 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Blocking and dilution buffer: 5% NFDM /TBST.
Anti-IL-8 antibody [EPR1116(2)] (ab110727) at 1/1000 dilution (unpurified) + IL8 recombinant protein at 10 µg
Predicted band size: 11 kDa
This product has been referenced in:
- Lee SY et al. Establishment of a chronic obstructive pulmonary disease mouse model based on the elapsed time after LPS intranasal instillation. Lab Anim Res 34:1-10 (2018). Read more (PubMed: 29628971) »
- Prattichizzo F et al. Short-term sustained hyperglycaemia fosters an archetypal senescence-associated secretory phenotype in endothelial cells and macrophages. Redox Biol 15:170-181 (2017). Read more (PubMed: 29253812) »