Recombinant Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)
![Western blot - Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)](https://www.abcam.com/ps/products/275/ab275255/Images/ab275255-453272-anti-il-8-antibody-epr22994-255-western-blot-wildtype-pc3-brefeldin-a-5-ugml-5-h-wildtype-pc3-lps-2-ugml-6-h-with.jpg "Western blot - Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22994-255] to IL-8 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free
See all IL-8 primary antibodies -
Description
Rabbit monoclonal [EPR22994-255] to IL-8 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: U-87 MG treated with 1µM Thapsigargin for 24 hours, whole cell lysate; Wild-type PC-3 LPS-treated (2 µg/ml, 6 h) with Brefeldin A (ab120299) (5 µg/ml, 5 h) cell lysate; His-tagged human recombiant IL-8 protein (aa21-99).
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General notes
ab275255 is the carrier-free version of ab235584.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22994-255 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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KO cell lines
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KO cell lysates
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
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| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa. |
Target
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Function
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
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Alternative names
- (Ala-IL-8)77 antibody
- (Ser-IL-8)72 antibody
- 9E3 antibody
see all
Images
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Western blot - Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)All lanes : Anti-IL-8 antibody [EPR22994-255] (ab235584) at 1/1000 dilution
Lane 1 : Wild-type PC-3 Brefeldin A (ab120299)-treated (5 μg/ml, 5 h) cell lysate
Lane 2 : Wild-type PC-3 LPS-treated (2 μg/ml, 6 h) with Brefeldin A (ab120299) (5 μg/ml, 5 h) cell lysate
Lane 3 : CXCL8 knockout PC-3 Brefeldin A (ab120299)-treated (5 μg/ml, 5 h) cell lysate
Lane 4 : CXCL8 knockout PC-3 LPS-treated (2 μg/ml, 6 h) with Brefeldin A (ab120299) (5 μg/ml, 5 h) cell lysate
Lane 5 : A431 cell lysate
Lane 6 : HCT116 cell lysate
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab235584).
Lanes 1 - 6: Merged signal (red and green). Green - ab235584 observed at 10 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab235584 was shown to react with IL-8 in wild-type PC-3 cells in Western blot with loss of signal observed in CXCL8 knockout sample. Wild-type PC-3 and CXCL8 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab235584 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)](https://www.abcam.com/ps/products/275/ab275255/Images/ab275255-407619-anti-il8-antibody-epr22994255-western-blot-il8.jpg)
Western blot - Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)Anti-IL-8 antibody [EPR22994-255] (ab235584) at 1/1000 dilution + His-tagged human recombiant IL-8 protein (aa21-99) 10 ng
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 11 kDaThis data was developed using ab235584, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes
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![Western blot - Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)](https://www.abcam.com/ps/products/275/ab275255/Images/ab275255-407618-anti-il8-antibody-epr22994255-western-blot-glioblastomaastrocytoma.jpg)
Western blot - Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)All lanes : Anti-IL-8 antibody [EPR22994-255] (ab235584) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lane 2 : U-87 MG treated with 1uM Thapsigargin for 24 hours, whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lane 2 : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 11 kDaThis data was developed using ab235584, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes
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![Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)](https://www.abcam.com/ps/products/275/ab275255/Images/ab275255-3-benefits-of-recombinant-antibodies.png)
Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab275255 has not yet been referenced specifically in any publications.
