Anti-IL-8 antibody [MM0219-7F11] (ab89336)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-IL-8 antibody [MM0219-7F11]
See all IL-8 primary antibodies -
DescriptionMouse monoclonal [MM0219-7F11] to IL-8
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Host speciesMouse
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Tested applicationsSuitable for: WB, IHC-Fr, Neutralising, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant full length protein corresponding to Human IL-8 aa 22-98.
Sequence:GAVLPRSAK ELRCQCIKTY SKPFHPKFIK ELRVIESGPH CANTEIIVKL SDGRELCLDP KENWVQRVVE KFLKRAENS
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityProtein G purified
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Purification notesThe IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 µm filtered solution.
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ClonalityMonoclonal
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Clone numberMM0219-7F11
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab89336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 11 kDa. | |
| IHC-Fr | 1/20 - 1/100. | |
| Neutralising | Use at an assay dependent concentration. | |
| Flow Cyt | 1/50 - 1/200. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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FunctionIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
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Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
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Post-translational
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
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Alternative names
- (Ala-IL-8)77 antibody
- (Ser-IL-8)72 antibody
- 9E3 antibody
see all
Images
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![Immunohistochemistry (Frozen sections) - Anti-IL-8 antibody [MM0219-7F11] (ab89336)](https://www.abcam.com/ps/products/89/ab89336/Images/ab89336-105032-anti-il-8-antibody-mm0219-7f11-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-IL-8 antibody [MM0219-7F11] (ab89336)Image from Negi S et al., PLoS One. 2012;7(1):e30415. Epub 2012 Jan 27. Fig 4.; doi:10.1371/journal.pone.0030415; January 27, 2012, PLoS ONE 7(1): e30415.Immunofluorescence analysis of Human pancreas (left panels), freshly isolated d0-islets (centre panels) and cultured d3-islets (right panels). IL8 was stained using ab89336 at 1/50 dilution.
Datasheets and documents
References
This product has been referenced in:
- Allen CL et al. Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing. PLoS One 8:e78441 (2013). Read more (PubMed: 24205233) »
- Negi S et al. Analysis of Beta-Cell Gene Expression Reveals Inflammatory Signaling and Evidence of Dedifferentiation following Human Islet Isolation and Culture. PLoS One 7:e30415 (2012). IHC-Fr ; Human . Read more (PubMed: 22299040) »
