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  1. Link

    il-8-antibody-mm0219-7f11-ab89336.pdf

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Cardiovascular Angiogenesis Cytokines Interleukin
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Anti-IL-8 antibody [MM0219-7F11] (ab89336)

  • Datasheet
Reviews (2)Q&A (1)References (2)

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Key features and details

  • Mouse monoclonal [MM0219-7F11] to IL-8
  • Suitable for: IHC-Fr, Neutralising, Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-IL-8 antibody [MM0219-7F11]
    See all IL-8 primary antibodies
  • Description

    Mouse monoclonal [MM0219-7F11] to IL-8
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, Neutralising, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human IL-8 aa 22-98.
    Sequence:

    GAVLPRSAK ELRCQCIKTY SKPFHPKFIK ELRVIESGPH CANTEIIVKL SDGRELCLDP KENWVQRVVE KFLKRAENS

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 µm filtered solution.
  • Clonality

    Monoclonal
  • Clone number

    MM0219-7F11
  • Isotype

    IgG1
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Cytokines
    • Interleukin
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human IL-8 protein (Active) (ab259397)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab89336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
Neutralising
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB (1)
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
Neutralising
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
Use at an assay dependent concentration.

Target

  • Function

    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P10145 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3576 Human
    • Omim: 146930 Human
    • SwissProt: P10145 Human
    • Unigene: 624 Human
    • Alternative names

      • (Ala-IL-8)77 antibody
      • (Ser-IL-8)72 antibody
      • 9E3 antibody
      • Beta thromboglobulin like protein antibody
      • C-X-C motif chemokine 8 antibody
      • CEF-4 antibody
      • chemokine, CXC motif, ligand 8 antibody
      • CXCL8 antibody
      • Emoctakin antibody
      • GCP-1 antibody
      • GCP/IL-8 protein I antibody
      • GCP/IL-8 protein II antibody
      • GCP/IL-8 protein III antibody
      • GCP/IL-8 protein IV antibody
      • GCP/IL-8 protein V antibody
      • GCP/IL-8 protein VI antibody
      • GCP1 antibody
      • Granulocyte chemotactic protein 1 antibody
      • IL-8 antibody
      • IL-8(1-77) antibody
      • IL-8(9-77) antibody
      • IL8 antibody
      • IL8/NAP1 form I antibody
      • IL8/NAP1 form II antibody
      • IL8/NAP1 form III antibody
      • IL8/NAP1 form IV antibody
      • IL8/NAP1 form V antibody
      • IL8/NAP1 form VI antibody
      • IL8_HUMAN antibody
      • Inteleukin 8 antibody
      • LECT antibody
      • LUCT antibody
      • Lymphocyte-derived neutrophil-activating factor antibody
      • LYNAP antibody
      • MDNCF antibody
      • MDNCF-b antibody
      • MDNCF-c antibody
      • MONAP antibody
      • Monocyte derived neutrophil activating peptide antibody
      • Monocyte derived neutrophil chemotactic factor antibody
      • Monocyte-derived neutrophil chemotactic factor antibody
      • Monocyte-derived neutrophil-activating peptide antibody
      • NAF antibody
      • NAP 1 antibody
      • NAP-1 antibody
      • NAP1 antibody
      • Neutrophil activating peptide 1 antibody
      • Neutrophil activating protein 1 antibody
      • Neutrophil-activating factor antibody
      • Neutrophil-activating protein 1 antibody
      • Protein 3 10C antibody
      • Protein 3-10C antibody
      • SCYB 8 antibody
      • SCYB8 antibody
      • Small inducible cytokine subfamily B member 8 antibody
      • T cell chemotactic factor antibody
      • T-cell chemotactic factor antibody
      • TSG 1 antibody
      • TSG1 antibody
      see all

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (2)

    Publishing research using ab89336? Please let us know so that we can cite the reference in this datasheet.

    ab89336 has been referenced in 2 publications.

    • Allen CL  et al. Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing. PLoS One 8:e78441 (2013). PubMed: 24205233
    • Negi S  et al. Analysis of Beta-Cell Gene Expression Reveals Inflammatory Signaling and Evidence of Dedifferentiation following Human Islet Isolation and Culture. PLoS One 7:e30415 (2012). IHC-Fr ; Human . PubMed: 22299040

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

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    1-2 of 2 Abreviews

    Western blot abreview for Anti-IL8 antibody [MM0219-7F11]

    Average
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (AGS)
    Specification
    AGS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 02 2013

    Immunohistochemistry (Resin sections) abreview for Anti-IL8 antibody [MM0219-7F11]

    Poor
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Resin sections)
    Sample
    Human Tissue sections (Nasal polyp and tonsil)
    Specification
    Nasal polyp and tonsil
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 05 2010

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