Recombinant Anti-IL-8 antibody [O-IL8-15] - Mouse IgG2a (ab282000)
![Western blot - Anti-IL-8 antibody [O-IL8-15] - Mouse IgG2a (ab282000)](https://www.abcam.com/ps/products/282/ab282000/Images/ab282000-467662-anti-il8-antibody-oil815msigg2a-western-blot-untreated-u87-mg.jpg "Western blot - Anti-IL-8 antibody [O-IL8-15] - Mouse IgG2a (ab282000)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [O-IL8-15] to IL-8 - Mouse IgG2a
- Suitable for: WB, Flow Cyt (Intra), ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-IL-8 antibody [O-IL8-15] - Mouse IgG2a
See all IL-8 primary antibodies -
Description
Mouse monoclonal [O-IL8-15] to IL-8 - Mouse IgG2a -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt (Intra), ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: U-87 MG treated with 1uM thapsigargin for 24 h whole lysates. ICC/IF: U-87 MG treated with 1uM thapsigargin for 24 h whole lysates. Flow Cyt-intra: U-87 MG treated with 1uM thapsigargin for 24 h whole lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This antibody was developed by the Structural Genomics Consortium and manufactured by Abcam.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
O-IL8-15 -
Isotype
IgG2a -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Dyes/Markers
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab282000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 11 kDa.
|
|
| Flow Cyt (Intra) |
1/1000.
|
|
| ICC/IF |
1/50.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 11 kDa. |
|
Flow Cyt (Intra)
1/1000. |
|
ICC/IF
1/50. |
Target
-
Function
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
-
Alternative names
- (Ala-IL-8)77 antibody
- (Ser-IL-8)72 antibody
- 9E3 antibody
see all
Images
-
Western blot - Anti-IL-8 antibody [O-IL8-15] - Mouse IgG2a (ab282000)All lanes : Anti-IL-8 antibody [O-IL8-15] - Mouse IgG2a (ab282000) at 1/1000 dilution
Lane 1 : Untreated U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lane 2 : U-87 MG treated with 1uM thapsigargin for 24 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 11 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes
-
![Immunocytochemistry/ Immunofluorescence - Anti-IL-8 antibody [O-IL8-15] - Mouse IgG2a (ab282000)](https://www.abcam.com/ps/products/282/ab282000/Images/ab282000-2-anti-il8-antibody-oil815msigg2a-immunocytochemistry-u87-mg.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL-8 antibody [O-IL8-15] - Mouse IgG2a (ab282000)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-87 MG cells labelling IL-8 with ab282000 at 1/50 (22.9 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in U-87 MG cells treated with thapsigargin (1uM) for 24 hours.
ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
-
![Flow Cytometry (Intracellular) - Anti-IL-8 antibody [O-IL8-15] - Mouse IgG2a (ab282000)](https://www.abcam.com/ps/products/282/ab282000/Images/ab282000-3-anti-il8-antibody-oil815msigg2a-flow-cytometry-u87-mg.jpg)
Flow Cytometry (Intracellular) - Anti-IL-8 antibody [O-IL8-15] - Mouse IgG2a (ab282000)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) treated with 1uM thapsigargin for 24h (Right) / Untreated control (Left) cells labelling IL-8 with ab282000 at 1/1000 dilution (0.1ug). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
-
![Anti-IL-8 antibody [O-IL8-15] - Mouse IgG2a (ab282000)](https://www.abcam.com/ps/products/282/ab282000/Images/ab282000-4-Benefits-of-Recombinant-antibodies.jpg)
Anti-IL-8 antibody [O-IL8-15] - Mouse IgG2a (ab282000)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab282000 has not yet been referenced specifically in any publications.