FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Constituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.25 - 1 µg/ml.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 15 kDa.|
FunctionSupports IL-2 independent and IL-4 independent growth of helper T-cells.
Sequence similaritiesBelongs to the IL-7/IL-9 family.
- Information by UniProt
- Cytokine P40 antibody
- Homolog of mouse T cell and mast cell growth factor 40 antibody
- HP40 antibody
Western blot analysis of varying levels of Recombinant murine IL-9 protein using ab271260 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant IL-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Murine IL-9was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271260. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine IL-9 protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271260 has not yet been referenced specifically in any publications.