Anti-IL-9R antibody [4A11H2] (ab233757)
Key features and details
- Mouse monoclonal [4A11H2] to IL-9R
- Suitable for: WB, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-IL-9R antibody [4A11H2]
See all IL-9R primary antibodies -
Description
Mouse monoclonal [4A11H2] to IL-9R -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human IL-9R aa 1-300. Expressed in E.coli
Database link: Q01113 -
Positive control
- WB: Recombinant human IL9R (AA: extra 41-270); C6, PC-3 and IL9R (AA: extra 41-270)-hIgGFc transfected HEK-293 whole cell lysate. IHC-P: Human cervical cancer and ovarian cancer tissue. Flow Cyt: Ramos cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4A11H2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000.
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Flow Cyt |
1/200 - 1/400.
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Notes |
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WB
1/500 - 1/2000. |
Flow Cyt
1/200 - 1/400. |
Target
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Function
This is a receptor for interleukin-9. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Cell membrane. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3581 Human
- Entrez Gene: 24500 Rat
- Omim: 300007 Human
- SwissProt: Q01113 Human
- Unigene: 406228 Human
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Alternative names
- CD129 antibody
- IL-9 receptor antibody
- IL-9R antibody
see all
Images
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Anti-IL-9R antibody [4A11H2] (ab233757) at 1/500 dilution + Recombinant human IL9R protein
Developed using the ECL technique. -
All lanes : Anti-IL-9R antibody [4A11H2] (ab233757) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : IL9R (AA: extra 41-270)-hIgGFc transfected HEK-293 whole cell lysate
Developed using the ECL technique. -
All lanes : Anti-IL-9R antibody [4A11H2] (ab233757) at 1/500 dilution
Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Developed using the ECL technique. -
Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling IL9R with ab233757 at 1/200 dilution (green) compared with a negative control (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab233757 has been referenced in 1 publication.
- Sun Y et al. Decreased placental IL9 and IL9R in preeclampsia impair trophoblast cell proliferation, invasion, and angiogenesis. Hypertens Pregnancy N/A:1-8 (2020). PubMed: 32329646