• Product name
    Anti-IL1 alpha antibody [AS5]
    See all IL1 alpha primary antibodies
  • Description
    Mouse monoclonal [AS5] to IL1 alpha
  • Host species
  • Specificity
    Recognizes human IL-1 alpha. Does not recognize mouse IL-1 alpha, human IL-1 beta or a panel of other human cytokines.
  • Tested applications
    Suitable for: IHC-P, IP, ELISA, Neutralising, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant IL1 alpha protein (Human).



Our Abpromise guarantee covers the use of ab17281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. (see Abreview)
IP Use at an assay dependent concentration. This antibody has been used to immunoprecipitate >90% human IL-1 (in solution using a double-antibody method).
ELISA Use a concentration of 2 µg/ml. This antibody has been used as a solution phase antibody in a two site ELISA with biotin conjugated IL-1 alpha antibody. Typically the ELISA has a working range of 0-10000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 µl. Recovery of human IL-1 alpha in human serum is typically >85% in the ELISA.
Neutralising Use a concentration of 25 mg/ml. This antibody has been used to neutralize >95% of the biological activity of human IL-1 at 0.5 mg/ml.
IHC (PFA fixed) Use a concentration of 1 - 10 µg/ml. This antibody has been used to stain human IL-1 alpha expressed in mononuclear cells, following fixation with 4% paraform in PBS for 10 minutes at 4oC plus 0.1% saponin 20 minutes at RT.


  • Function
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similarities
    Belongs to the IL-1 family.
  • Domain
    The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localization
    Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAF antibody
    • FAF antibody
    • Hematopoietin 1 antibody
    • Hematopoietin-1 antibody
    • IL 1 alpha antibody
    • IL 1A antibody
    • IL-1 alpha antibody
    • Il-1a antibody
    • IL1 ALPHA antibody
    • IL1 antibody
    • IL1A antibody
    • IL1A_HUMAN antibody
    • IL1F1 antibody
    • Interleukin 1 alpha antibody
    • Interleukin-1 alpha antibody
    • Interleukin1 alpha antibody
    • LAF antibody
    • LEM antibody
    • Preinterleukin 1 alpha antibody
    • Pro interleukin 1 alpha antibody
    see all


  • ab17281 staining IL1 alpah in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using EDTA buffer pH 9.0. Samples were then blocked with 10% serum for 30 minutes at 21°C followed by incubation with the primary antibody at a 1/200 dilution for 30 minutes at 21°C. A undiluted HRP-conjugated goat polyclonal was used as secondary antibody.

    See Abreview


This product has been referenced in:
  • Zhang X  et al. Expression of TP53 and IL-1a in unicystic ameloblastoma predicts the efficacy of marsupialization treatment. Medicine (Baltimore) 97:e9795 (2018). Read more (PubMed: 29419674) »
  • Zhang X  et al. H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis. Nat Commun 8:14799 (2017). Read more (PubMed: 28300060) »

See all 2 Publications for this product

Customer reviews and Q&As

I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

We look forward to your review. If the results are still negative, please let us know...

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Thanks for your enquiry.

Its estimated we will havemore vialsof ab17281 in stock by Monday 6, 2012.

I hope this is helpful. Please contact us again if you have any further questions.

Thanks for the reply.

A new vial of ab17281 is on its way to you, Your new order number if *.

If there is anything else I can help you with, or if you have any issues with the new vial of antibody, then please let me know.

Thank you for your reply.

Unfortunately we do not have any other lots of this antibody available, so maybe it would be better to send a different IL-1 alpha antibody for you try.Or if you wish to give ab17281 another try, I’m happy to se...

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Thanks for letting me know.

I am sorry that increasing the primary antibody concentration did not work for you. Therefore I think the best way to move forward on this one, is to either send you a new aliquot of ab17281, in case there was somet...

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Thank you for contacting Abcam.

I am sorry about the issues you have been having with ab17281 in IHC-P. If the protocol variations we talked about today do not prove to be successful, then please let me know as the antibody is covered under ou...

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Thank you once again for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investig...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH 9.0
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C

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Verified customer

Submitted Jan 11 2011


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