Anti-IL-1 beta antibody (ab2105)
Key features and details
- Rabbit polyclonal to IL-1 beta
- Suitable for: RIA, ELISA, ICC/IF, IHC-Fr, Functional Studies, WB, IP, Neutralising, Flow Cyt, IHC-P, IHC-FoFr
- Reacts with: Human
- Isotype: IgG
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-IL-1 beta antibody
See all IL-1 beta primary antibodies -
Description
Rabbit polyclonal to IL-1 beta -
Host species
Rabbit -
Tested applications
Suitable for: RIA, ELISA, ICC/IF, IHC-Fr, Functional Studies, WB, IP, Neutralising, Flow Cyt, IHC-P, IHC-FoFrmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates -
Immunogen
Recombinant full length protein corresponding to Human IL-1 beta aa 100 to the C-terminus. Produced in E.coli. MW of recombinant IL-1 beta 17kDa, mature chain without propeptide.
Database link: P01584 -
Positive control
- IHC-P: human medullary lymph node
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General notes
caution, F(c) domain of rabbit IgG may interact with cells non-specifically.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
IgG from whole rabbit serum purified by DEAE fractionation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
RIA |
Use at an assay dependent concentration.
|
|
ELISA | (1) |
Use at an assay dependent concentration.
|
ICC/IF | (4) |
Use at an assay dependent concentration.
|
IHC-Fr | (2) |
Use at an assay dependent concentration.
|
Functional Studies |
Use at an assay dependent concentration.
|
|
WB | (6) |
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa.
1/1000 - 1/2000 (antigen - supernatants or lysates of 2 x 106 endotoxin-stimulated human PBMC. Denatured 31kDa precursor IL-1beta will be recognized, but often migrates as a 35 kDa band). |
IP |
Use at an assay dependent concentration.
1/400 - 1/800 (pre-clearing with a non-specific rabbit IgG is helpful to reduce background). |
|
Neutralising |
Use at an assay dependent concentration.
Neutralization of IL-1beta activity in bioassays 1/100 ( >4 hours incubation, normal rabbit IgG as negative control). ab2105 does not neutralize the biological activity of murine, rat or rabbit IL1 beta. |
|
Flow Cyt | (1) |
Use at an assay dependent concentration.
F(c) domain of rabbit IgG may interact with cells non-specifically! ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P | (9) |
Use at an assay dependent concentration.
|
IHC-FoFr |
Use at an assay dependent concentration. PubMed: 28726778
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Notes |
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RIA
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa. 1/1000 - 1/2000 (antigen - supernatants or lysates of 2 x 106 endotoxin-stimulated human PBMC. Denatured 31kDa precursor IL-1beta will be recognized, but often migrates as a 35 kDa band). |
IP
Use at an assay dependent concentration. 1/400 - 1/800 (pre-clearing with a non-specific rabbit IgG is helpful to reduce background). |
Neutralising
Use at an assay dependent concentration. Neutralization of IL-1beta activity in bioassays 1/100 ( >4 hours incubation, normal rabbit IgG as negative control). ab2105 does not neutralize the biological activity of murine, rat or rabbit IL1 beta. |
Flow Cyt
Use at an assay dependent concentration. F(c) domain of rabbit IgG may interact with cells non-specifically! ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 28726778 |
Target
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Function
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. -
Tissue specificity
Expressed in activated monocytes/macrophages (at protein level). -
Sequence similarities
Belongs to the IL-1 family. -
Post-translational
modificationsActivation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. -
Cellular localization
Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. - Information by UniProt
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Database links
- Entrez Gene: 3553 Human
- Omim: 147720 Human
- SwissProt: P01584 Human
- Unigene: 126256 Human
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Alternative names
- Catabolin antibody
- H1 antibody
- IFN beta inducing factor antibody
see all
Images
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Immunohistochemical analysis of Formalin-fixed paraffin-embedded human medullary lymph node sections labeling IL-1 beta with ab2105. Peroxidase goat anti-rabbit at 1/10,000 was used as the secondary antibody.
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ab2105 at 1/20 staining human liver tissue sections by IHC-P. The cells were paraformaldehyde fixed and a heat mediated antigen retrieval step was performed. The tissue was incubated with the tissue overnight at room temperature. A biotinylated donkey anti-rabbit IgG was used as the secondary antibody and this was detected using streptavidin HRP.
Protocols
Datasheets and documents
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Datasheet download
References (175)
ab2105 has been referenced in 175 publications.
- Suliman HB et al. Nuclear respiratory factor-1 negatively regulates TGF-β1 and attenuates pulmonary fibrosis. iScience 25:103535 (2022). PubMed: 34977500
- Meng L et al. METTL14 suppresses pyroptosis and diabetic cardiomyopathy by downregulating TINCR lncRNA. Cell Death Dis 13:38 (2022). PubMed: 35013106
- Evangelou K et al. Pulmonary infection by SARS-CoV-2 induces senescence accompanied by an inflammatory phenotype in severe COVID-19: possible implications for viral mutagenesis. Eur Respir J 60:N/A (2022). PubMed: 35086840
- Hu M et al. Effect of α-tocopherol in alleviating the lipopolysaccharide-induced acute lung injury via inhibiting nuclear factor kappa-B signaling pathways. Bioengineered 13:3958-3968 (2022). PubMed: 35112986
- Pu J et al. Immunohistochemical analysis of pyroptosis-related protein expression in IgG4-related sialadenitis. J Oral Pathol Med 51:388-394 (2022). PubMed: 35218237