Mouse monoclonal  to IL1 Receptor I / IL-1R-1
ab115497 reacts with cell surface and soluble human IL1 Receptor I. The specific epitope is unknown. ab115497 does not block binding of IL-1 to the receptor, and does not cross-react with receptors for human IL-2 or IL-4.
Recombinant fragment corresponding to Human IL1 Receptor I/ IL-1R-1 (extracellular).
Human Placenta tissue.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Previously labelled as IL1 Receptor I.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration. Concentrations of 20-30 ng/ml will bind radiolabeled IL1 Receptor I in a radioimmunoassay format.
Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
Belongs to the interleukin-1 receptor family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 TIR domain.
ab115497, at 10 µg/ml, staining IL1 Receptor I / IL-1R-1 in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry using a biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.