Anti-IL1 Receptor I / IL-1R-1 antibody (ab106278)

Overview

  • Product name
    Anti-IL1 Receptor I / IL-1R-1 antibody
    See all IL1 Receptor I / IL-1R-1 primary antibodies
  • Description
    Rabbit polyclonal to IL1 Receptor I / IL-1R-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human IL1 Receptor I/ IL-1R-1 aa 18-241.

  • Positive control
    • Human fetal spleen lysate. HeLa cell lysate. Human fetal skin tissue.
  • General notes

    Previously labelled as IL1 Receptor I. 

Properties

Applications

Our Abpromise guarantee covers the use of ab106278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24950657
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 65 kDa).
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
  • Sequence similarities
    Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen CD121a antibody
    • CD121 antigen-like family member A antibody
    • CD121a antibody
    • CD121a antigen antibody
    • D2S1473 antibody
    • IL 1 antibody
    • IL 1R 1 antibody
    • IL 1R alpha antibody
    • IL 1RT1 antibody
    • IL-1R-1 antibody
    • IL-1R-alpha antibody
    • IL-1RT-1 antibody
    • IL-1RT1 antibody
    • IL1 inhibitor antibody
    • IL1 Receptor I antibody
    • IL1R antibody
    • IL1R1 antibody
    • IL1R1_HUMAN antibody
    • IL1RA antibody
    • IL1RN antibody
    • IL1RT1 antibody
    • Interleukin 1 receptor alpha type 1 antibody
    • Interleukin 1 receptor antagonist protein (IRAP) antibody
    • Interleukin 1 receptor type I antibody
    • Interleukin receptor 1 antibody
    • Interleukin-1 receptor alpha antibody
    • Interleukin-1 receptor type 1 antibody
    • Interleukin-1 receptor type I antibody
    • p80 antibody
    see all

Images

  • ab106278 staining interleukin 1 receptor 1 antibody in foetal epithelial skin tissue by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antibody dilution 1/100.

  • All lanes : Anti-IL1 Receptor I / IL-1R-1 antibody (ab106278) at 1/500 dilution

    Lane 1 : Human fetal spleen lysate
    Lane 2 : HeLa cell lysate

    Predicted band size: 65 kDa

References

This product has been referenced in:
  • Paish HL  et al. Fibroblasts Promote Inflammation and Pain via IL-1a Induction of the Monocyte Chemoattractant Chemokine (C-C Motif) Ligand 2. Am J Pathol 188:696-714 (2018). IHC ; Human . Read more (PubMed: 29248462) »
  • Lu Q  et al. Comprehensive microRNA profiling reveals potential augmentation of the IL1 pathway in rheumatic heart valve disease. BMC Cardiovasc Disord 18:53 (2018). Read more (PubMed: 29548280) »

See all 5 Publications for this product

Customer reviews and Q&As

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ab106278 : sodium citrate buffer (10 mM Sodium citrate, pH 6.0), boil for 15 minutes.

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Colon, Dorsal Root Ganglia)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Colon, Dorsal Root Ganglia
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Oct 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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