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Rabbit polyclonal to IL1 Receptor I
Predicted to work with:
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human IL1 Receptor I.
This antibody gave a positive signal in the following human tissue lysates: Heart; Placenta; Lung.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 65 kDa).
Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 TIR domain.
Information by UniProt
Antigen CD121a antibody
CD121 antigen-like family member A antibody
Western blot - Anti-IL1 Receptor I antibody (ab94393)
All lanes :
Anti-IL1 Receptor I antibody (ab94393) at 1 µg/ml
Lane 1 :
Human heart tissue lysate - total protein (
Lane 2 :
Human placenta tissue lysate - total protein (
Lane 3 :
Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
73 kDa (
why is the actual band size different from the predicted?
Additional bands at:
92 kDa. We are unsure as to the identity of these extra bands.
IL1 Receptor I contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"