Overview

  • Product name

    Anti-IL1 Receptor I/IL-1R-1 antibody
    See all IL1 Receptor I/IL-1R-1 primary antibodies
  • Description

    Rabbit polyclonal to IL1 Receptor I/IL-1R-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human IL1 Receptor I/IL-1R-1 aa 18-241.

  • Positive control

    • Human fetal spleen lysate. HeLa cell lysate. Human fetal skin tissue.
  • General notes

    Previously labelled as IL1 Receptor I. 

Properties

Applications

Our Abpromise guarantee covers the use of ab106278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24950657
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 65 kDa).
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
  • Sequence similarities

    Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen CD121a antibody
    • CD121 antigen-like family member A antibody
    • CD121a antibody
    • CD121a antigen antibody
    • D2S1473 antibody
    • IL 1 antibody
    • IL 1R 1 antibody
    • IL 1R alpha antibody
    • IL 1RT1 antibody
    • IL-1R-1 antibody
    • IL-1R-alpha antibody
    • IL-1RT-1 antibody
    • IL-1RT1 antibody
    • IL1 inhibitor antibody
    • IL1 Receptor I antibody
    • IL1R antibody
    • IL1R1 antibody
    • IL1R1_HUMAN antibody
    • IL1RA antibody
    • IL1RN antibody
    • IL1RT1 antibody
    • Interleukin 1 receptor alpha type 1 antibody
    • Interleukin 1 receptor antagonist protein (IRAP) antibody
    • Interleukin 1 receptor type I antibody
    • Interleukin receptor 1 antibody
    • Interleukin-1 receptor alpha antibody
    • Interleukin-1 receptor type 1 antibody
    • Interleukin-1 receptor type I antibody
    • p80 antibody
    see all

Images

  • ab106278 staining IL-1 Receptor 1 / IL-1R-1 antibody in foetal epithelial skin tissue by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antibody dilution 1/100.

  • All lanes : Anti-IL1 Receptor I/IL-1R-1 antibody (ab106278) at 1/500 dilution

    Lane 1 : Human fetal spleen lysate
    Lane 2 : HeLa cell lysate

    Predicted band size: 65 kDa

References

This product has been referenced in:

  • Paish HL  et al. Fibroblasts Promote Inflammation and Pain via IL-1a Induction of the Monocyte Chemoattractant Chemokine (C-C Motif) Ligand 2. Am J Pathol 188:696-714 (2018). IHC ; Human . Read more (PubMed: 29248462) »
  • Lu Q  et al. Comprehensive microRNA profiling reveals potential augmentation of the IL1 pathway in rheumatic heart valve disease. BMC Cardiovasc Disord 18:53 (2018). Read more (PubMed: 29548280) »
See all 9 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (spleen)
Permeabilization
Yes - 0.5% triton
Specification
spleen
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Nov 16 2018

Answer

Thank you for contacting us and your interest in our products. xxxx passed on your query to the Scientific Support team and I will endeavour to answer yourquestion as bestI can.


We have a couple of antibodies which may be suitable for the experiments you are hoping to perform. https://www.abcam.com/IL1-beta-antibody-Biotin-ab106034.htmlis a rabbit polyclonal antibody, directed against mouse IL 1 beta. This antibody has been tested in staining paraffin embedded tissue sections. The application and species you wish to use are therefore covered by the Abpromise. The antibody is however biotin conjugated.


https://www.abcam.com/IL1-beta-antibody-HRP-ab106035.htmlis an HRP
conjugated rabbit polyclonal antibody and again has been tested in staining of paraffin embedded tissue sections. This would again be covered by the Abpromise for the application and speciesyou are looking to use. More information about the Abpromise can be found from the following link:


https://www.abcam.com/abpromise


Alternatively, https://www.abcam.com/IL1-beta-antibody-ab9722.htmlis a quite well characterised rabbit polyclonal antibody directed against mouse IL 1 beta. This antibody has to our knowledge not been used in staining of paraffin embedded tissue yet. However this does not mean that it will not work, simply that we have not tested it so therefore do not know. As we are unable to guarantee such antibodies to work, we offer a testing discount scheme.


This offer means that if you purchase and test https://www.abcam.com/IL1-beta-antibody-ab9722.htmlin IHC on paraffin embedded sections and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalog. More information on this offer can be found from the following link:


https://www.abcam.com/collaboratordiscount


If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of https://www.abcam.com/IL1-beta-antibody-ab9722.html.


I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Answer

ab106278 : sodium citrate buffer (10 mM Sodium citrate, pH 6.0), boil for 15 minutes.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Colon, Dorsal Root Ganglia)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Colon, Dorsal Root Ganglia
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 19 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up