Anti-IL1 Receptor I/IL-1R-1 antibody (ab106278)
Key features and details
- Rabbit polyclonal to IL1 Receptor I/IL-1R-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL1 Receptor I/IL-1R-1 antibody
See all IL1 Receptor I/IL-1R-1 primary antibodies -
Description
Rabbit polyclonal to IL1 Receptor I/IL-1R-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human IL1 Receptor I/IL-1R-1 aa 18-241.
Sequence:LEA DKCKEREEKI ILVSSANEID VRPCPLNPNE HKGTITWYKD DSKTPVSTEQ ASRIHQHKEK LWFVPAKVED SGHYYCVVRN SSYCLRIKIS AKFVENEPNL CYNAQAIFKQ KLPVAGDGGL VCPYMEFFKN ENNELPKLQW YKDCKPLLLD NIHFSGVKDR LIVMNVAEKH RGNYTCHASY TYLGKQYPIT RVIEFITLEE NKPTRPVIVS PANETMEVDL G
Database link: P14778 -
General notes
Previously labelled as IL1 Receptor I.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab106278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/200 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 65 kDa). | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3554 Human
- Omim: 147810 Human
- SwissProt: P14778 Human
- Unigene: 701982 Human
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Alternative names
- Antigen CD121a antibody
- CD121 antigen-like family member A antibody
- CD121a antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL1 Receptor I/IL-1R-1 antibody (ab106278)
ab106278 used at 1/100 dilution staining IL-1 Receptor 1 / IL-1R-1 in fetal human epithelial skin tissue by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody (ab106278)All lanes : Anti-IL1 Receptor I/IL-1R-1 antibody (ab106278) at 1/500 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 65 kDa
Protocols
References (16)
ab106278 has been referenced in 16 publications.
- Deng Y et al. Estradiol/GPER affects the integrity of mammary duct-like structures in vitro. Sci Rep 10:1386 (2020). PubMed: 31992771
- Reis AS et al. Inflammasome activation and IL-1 signaling during placental malaria induce poor pregnancy outcomes. Sci Adv 6:eaax6346 (2020). PubMed: 32181339
- Liang Y et al. Activation of the IL-1ß/KLF2/HSPH1 pathway promotes STAT3 phosphorylation in alveolar macrophages during LPS-induced acute lung injury. Biosci Rep 40:N/A (2020). PubMed: 32091104
- Lee KE et al. The extracellular vesicle of gut microbial Paenalcaligenes hominis is a risk factor for vagus nerve-mediated cognitive impairment. Microbiome 8:107 (2020). PubMed: 32669127
- Dosh RH et al. Interleukin 1 is a key driver of inflammatory bowel disease-demonstration in a murine IL-1Ra knockout model. Oncotarget 10:3559-3575 (2019). PubMed: 31191826
